LOCUS       sequence-109335-         337 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A lentiviral dual fluorescent protein reporter, HypoxCR, detects 
            hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Le A, Stine ZE, Nguyen C, Afzal J, Sun P, Hamaker M, Siegel NM, Gouw
            AM, Kang BH, Yu SH, Cochran RL, Sailor KA, Song H, Dang CV
  TITLE     Tumorigenicity of hypoxic respiring cancer cells revealed by a 
            hypoxia-cell cycle dual reporter.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Aug 11. pii: 201402012.
  PUBMED    25114222
REFERENCE   2  (bases 1 to 337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2014 Aug 11. pii: 201402012."
COMMENT     Sequence Label: HypoxCR
FEATURES             Location/Qualifiers
     source          1..337
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..335)
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="PSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELSAG
                     LSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAEKR
                     RKAL"
ORIGIN
        1 tctttgtagt cgagggcttt ccgtctcttc tcggcgacct ctttccagta ttggctacta
       61 gggttctcct tgatcatcag gtcgaagctt tcttgtgtca caccgcccaa gttcttgttc
      121 tcggagctct ccggcacgat cacgcctgga gagcttgtag tgctggtgag atgatcgttt
      181 ctgtgcttgc gcttgctcag tccggcgctc aattcgttct cgcgacccac gaggctgccg
      241 ctggcggatg gttggatcat tttgagggtc cggcgaggca cactggagtt tttgatgttt
      301 tccttaatct cctcctgctt ttgcttcatg gatgggt
//
