LOCUS       sequence-109338-         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CrBlue is a synthetic chimera comprising the blue opsin backbone 
            with the intracellular loops and the C terminus of the jellyfish 
            opsin, created to introduce spectral selectivity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Karunarathne WK, Giri L, Kalyanaraman V, Gautam N
  TITLE     Optically triggering spatiotemporally confined GPCR activity in a 
            cell and programming neurite initiation and extension.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1565-74. doi: 
            10.1073/pnas.1220697110. Epub 2013 Mar 11.
  PUBMED    23479634
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220697110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-04-23- 23"; volume: "110"; issue: "17"; pages: 
            "E1565-74"
COMMENT     Sequence Label: CrBlue ---- pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..61
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        42..60
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tggctactag agaacccact gccttactgg cttatcgaaa ttaatacgac tcactatagg
       61 gagacccaag ctggctagcg tttaaactta agcttggtac cgagctcgga tccactagtc
      121 cagtgtggtg gaattcaatt ccaccatgcg caagatgtcc gaggaggagt tctacctgtt
      181 caagaacatc tcgagcgtcg gcccttggga cgggccccag taccacatcg ctcctgtctg
      241 ggctttctac ctccaggctg ctttcatggg gaccgtgttc ctcatcggtt tccctctgaa
      301 tgccatggtg ctcgtggcct tttaccgctt acgacataaa ttggcttttt taaactacat
      361 cctggtgaac gtgtccttcg gtggattcct gctgtgtatc ttctccgtgt tccctgtgtt
      421 cgtggctagc tgcaacggtt acttcgtgtt cggacgtcac gtgtgtgctc tggagggttt
      481 cctaggtacc gtggctggtc tggtcaccgg gtggtctctg gccttcctgg ccttcgaacg
      541 ctacatcgtc atatgcagac ctttcgtggc aactgcgatt cacggatcaa tgagaaatgc
      601 gttaaccgta gtactggcta cctggacaat cggtatcggt gtatcgatcc ctcccttctt
      661 cggttggtct cgcttcattc cggaaggcct tcagtgcagc tgtggccctg actggtacac
      721 cgtgggtaca aagtaccgat cggagagcta cacctggttc ctgttcatct tctgcttcat
      781 cgtgcctctg agcctgatct gcttctccta caccttggtt caaggagaga tgaagaatat
      841 gcgaggtcgt gcagcacagt gttgg
//
