LOCUS       sequence-109339-       11115 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A lentiviral vector for the expression of mouse MYRF under 
            doxycyline control..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11115)
  AUTHORS   Najm FJ, Lager AM, Zaremba A, Wyatt K, Caprariello AV, Factor DC, 
            Karl RT, Maeda T, Miller RH, Tesar PJ
  TITLE     Transcription factor-mediated reprogramming of fibroblasts to 
            expandable, myelinogenic oligodendrocyte progenitor cells.
  JOURNAL   Nat Biotechnol. 2013 May;31(5):426-33. doi: 10.1038/nbt.2561. Epub 
            2013 Apr 14.
  PUBMED    23584611
REFERENCE   2  (bases 1 to 11115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2561"; 
            journalName: "Nat Biotechnol"; date: "2013-05"; volume: "31"; issue:
            "5"; pages: "426-33"
COMMENT     Sequence Label: pLVX-Tight-Puro_MYRF
FEATURES             Location/Qualifiers
     source          1..11115
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2206..2520
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2214..2232
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2250..2268
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2285..2303
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2321..2339
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2357..2375
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2392..2410
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2428..2446
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2493..2517
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        5900..6399
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5935..5958)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6332..6351
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6420..7019
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6420..6439)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6916..6936
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7037..7625
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7090..7110)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7508..7519)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7833..8466
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8595..8611)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8595..8611)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8608..8630)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8619..8635
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8643..8649,8650..8667,8668..8673))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8688..8709
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8826..8843)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8997..9582)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9077..9096)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9753..10544,10545..10613))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10376..10395
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10614..10718)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10766..10900
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10803..10822)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10857..10876
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat
     2221 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt
     2281 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
     2341 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
     2401 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
     2461 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2521 tggagaagga tccgcggccg caccatggag gtggtggacg agaccgaagc gctgcagcgc
     2581 ttcttcgaag gccatgacat cagcggtgcc ctggagccct ccaatataga caccagtatc
     2641 ctggaggagt acattggcaa agaggacgcc tctgatctct gcttccctga gatctctgca
     2701 ccagccagca ctgcctcctt cccccacggg ccaccggcca ttcccggctc cagcgggctc
     2761 caccatctga gcccccctgg gagcggacca tcccctgggc gccatggccc cctcccaccc
     2821 ccgacctacg gcaccccact caactgcaac aacaacaacg gcatgggcac cgcccctaag
     2881 cccttcctgg ggggctctgg gcctcccatc aaagcagagc caaaggcccc ctatgcccca
     2941 ggcacactgc cagactcgcc cccagactca ggctctgagg cctactcccc tcagcaggtg
     3001 aatgaccccc atcttctacg caccataacc ccggagactc tatgccacgt gggagtttct
     3061 tcccgcctgg agcacccgcc cccacctcca gcccacctgc caggcccacc accaccgcca
     3121 cctcccccac ctcactaccc tgtcctgcaa cgggacctct acatgaaggc tgagccccct
     3181 gtaccccctt atgctgccat ggggccgggt ctggtgcccc ccgagctcca tcacacccag
     3241 cagacccagg tgctacacca gctgctgcaa cagcatggag ctgaactccc tccacacccc
     3301 tctaagaaga ggaagcactc tgaatcaccc cccaacaccc tcaatgccca aatgctgaac
     3361 ggaatgatca agcaggaacc tgggactgtc acagccttgc ctccacaccc tgccagagcc
     3421 ccatcccctc catggcctcc tcaaggccca ctgtcacctg gcactggatc cttgcccctc
     3481 agcattgccc gagcccagac tccaccctgg cacccgccag gtgcaccctc cccaggcctc
     3541 ctgcaggaca gtgacagcct cagtggctcc tatttggacc ccaactacca atccatcaaa
     3601 tggcagccgc atcagcagaa caagtgggcg accctgtatg acgctaacta caaggagctg
     3661 cctatgctca cctatcgtgt ggacgctgac aagggcttca acttttccgt gggcgacgat
     3721 gcttttgtgt gccagaagaa gaaccacttc caggtgaccg tgtacatcgg catgctgggg
     3781 gagcccaagt acgtcaagac accggaaggc ctcaagcccc tggactgctt ctatctgaag
     3841 ctgcatggtg tgaagctaga ggccctgaac cagtctatca acattgagca gtcacagtca
     3901 gacagaagca agaggccctt caaccccgtc acggtcaatc ttccccctga gcaggtcaca
     3961 aaagtgaccg tggggcggct ccatttcagt gagaccactg ccaacaacat gcgcaagaag
     4021 ggcaagccca accctgacca gaggtatttc atgctggtgg tggccctcca ggcacatgca
     4081 cagaaccaga actacacact ggcagcccag atctcagagc gtatcattgt gcgggcctct
     4141 aacccaggcc agtttgaaag tgacagtgac gtgctgtggc aacgggcgca gctgccagat
     4201 acggtcttcc accatggccg tgtgggcatc aacaccgacc ggccagatga ggcattggtc
     4261 gtccacggca acgtgaaggt catgggttct cttatgcacc cttccgatct gcgggccaag
     4321 gagcacgtgc aggaggtgga caccaccgag cagctgaaga ggatctctcg gatgcggctg
     4381 gtgcactaca gatacaagcc tgagttcgct gctagcgcag gcattgaagc caccgcaccg
     4441 gagacaggtg tcatcgccca ggaagtgaag gagatcctgc ctgaagctgt gaaggacaca
     4501 ggggatgtag tctttgccaa tgggaaaacc atagagaact tccttgtagt gaacaaggag
     4561 cgaatcttca tggagaatgt gggggctgtg aaggagttat gcaaactgac agacaaccta
     4621 gagactcgca ttgatgagtt ggagcgatgg agccacaagc tggccaagct gcggcgcctt
     4681 gacagcctca agtcaactgg cagctcaggg gctttcagcc atgcagggag ccagtttagc
     4741 cgggcaggca gtgtccccca caaaaagagg ccccctaaac tggccaataa gtcatcgcca
     4801 gcggtcccag accaggcctg catcagtcag cgttttctgc agggaactat catagctctg
     4861 gtggtggtca tggccttcag cgtggtgtct atgtccacac tatatgtgct gagcctgcgc
     4921 tctgaagagg acctggtgga tgctgatggc aggtccagcc agagcttcgg gaccacacag
     4981 ctccgacagt cctccatgac caccggacta ccaggcacac agccctcttt gctgctggtt
     5041 accaagtcag cctcgggtcc agctctccgt gccttggacc tgtgctccag ccagccctgc
     5101 cccatcgtct gctgctctcc tcccgtctcc agtcctgcta cagatcctgc ccttggcccc
     5161 actcttactc ctactccaag ccccagctct aaccccaagc actcaggccc tggccagatg
     5221 gccccactgc cagtcaccaa catcagagcc aaatcctggg gcatctcagc taatggcatc
     5281 agctattcca agcattccaa gagcctggaa cctctggcca gtcctgtggt cccctttcct
     5341 ggagggcaga gcaagaccaa gaacagcccc agcttcaatc tccaaagtcg agcccgcaga
     5401 ggagccccgc aacccagccc aagccctgcc caattcacac agacccaggg ccagctagac
     5461 ccagcgccat ccctgacctc catccagctg ctggagaatt ccatgcctat cacttctcag
     5521 tactgtgtgc cagaaggtgc ttgcaggctt ggcaacttca cctaccacat ccctgtcagc
     5581 agcagcacac cactgcacct cagcctgacc ctgcagatga attcctccac ccctgtgtcc
     5641 gtggtactgt gcagcctgac atcggaggag gagccctgtg aggagggagg ctttttgcag
     5701 aggttccacc cgcatcagga cacccagggc acctctcatc agtggccagt aaccatcctg
     5761 tccttccgtg aattcacata ccacttccgg gtgacattgc tgggtcaggc caactgcagc
     5821 tcagaggcca tcgttcagcc agccaccgac tactacttcc acttctaccg cctgtgtgac
     5881 tagacgcgtg aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
     5941 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
     6001 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
     6061 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
     6121 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
     6181 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
     6241 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
     6301 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
     6361 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca
     6421 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     6481 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     6541 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     6601 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     6661 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     6721 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     6781 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     6841 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     6901 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     6961 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac
     7021 ggggcgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     7081 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     7141 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     7201 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     7261 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     7321 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     7381 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     7441 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     7501 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     7561 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     7621 cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc
     7681 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     7741 ggtttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     7801 tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact cccaacgaag
     7861 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa
     7921 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt
     7981 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca
     8041 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga
     8101 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg
     8161 ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct
     8221 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct
     8281 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     8341 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     8401 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     8461 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     8521 tatcagagag tgagaggcct tgacattgct agcgttttac cgtcgacctc tagctagagc
     8581 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     8641 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     8701 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     8761 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     8821 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8881 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8941 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     9001 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     9061 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     9121 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     9181 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     9241 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     9301 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     9361 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     9421 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     9481 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggttttttt
     9541 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     9601 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     9661 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9721 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9781 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9841 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9901 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9961 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    10021 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    10081 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    10141 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    10201 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    10261 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    10321 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    10381 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    10441 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    10501 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    10561 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    10621 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    10681 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    10741 cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata
    10801 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    10861 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
    10921 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctagtggt
    10981 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
    11041 gttaaatatg tactacaaac ttagtagttt ttaaagaaat tgtatttgtt aaatatgtac
    11101 tacaaactta gtagt
//
