LOCUS       sequence-109341-       10900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A lentiviral vector for the expression of mouse ST18 under 
            doxycyline control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10900)
  AUTHORS   Najm FJ, Lager AM, Zaremba A, Wyatt K, Caprariello AV, Factor DC, 
            Karl RT, Maeda T, Miller RH, Tesar PJ
  TITLE     Transcription factor-mediated reprogramming of fibroblasts to 
            expandable, myelinogenic oligodendrocyte progenitor cells.
  JOURNAL   Nat Biotechnol. 2013 May;31(5):426-33. doi: 10.1038/nbt.2561. Epub 
            2013 Apr 14.
  PUBMED    23584611
REFERENCE   2  (bases 1 to 10900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2561"; 
            journalName: "Nat Biotechnol"; date: "2013-05"; volume: "31"; issue:
            "5"; pages: "426-33"
COMMENT     Sequence Label: pLVX-Tight-Puro_ST18
FEATURES             Location/Qualifiers
     source          1..10900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2206..2520
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2214..2232
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2250..2268
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2285..2303
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2321..2339
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2357..2375
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2392..2410
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2428..2446
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2493..2517
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        5685..6184
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5720..5743)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6117..6136
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6205..6804
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6205..6224)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6701..6721
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6822..7410
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6875..6895)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7293..7304)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7618..8251
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8380..8396)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8380..8396)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8393..8415)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8404..8420
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8428..8434,8435..8452,8453..8458))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8473..8494
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8611..8628)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8782..9367)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8862..8881)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9538..10329,10330..10398))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10161..10180
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10399..10503)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10551..10685
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10588..10607)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10642..10661
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat
     2221 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt
     2281 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
     2341 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
     2401 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
     2461 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2521 tggagaagga tccaccatgg atgccgaggt tgaagataaa acgttgcata ctctttcaaa
     2581 aggaactgaa gttccaatgg attctctgat cccagagctc agggttccat atgattgctc
     2641 tatggcgaag aagaggagag ctgaagagca ggcatcggga gttccaatta acaagaggaa
     2701 atctctgcta atgaaacccc ggcactacag cccagacatg ggctgcaagg aaagcccaga
     2761 caacaggaac gaggatgatg gcctcctaga aacaaacgac catgccactg cagatgaaat
     2821 catggtaaaa tccatggatg aaactcttca tttacctgca caagacagct cactccagaa
     2881 gaaagaccaa tacacatgtt atccagagct catggtcaaa tctctggtgc acttaggcaa
     2941 atttgaggaa agtgagtctg tgcagaccac atgtgagaac ttaaatggca gcagtatcca
     3001 gtctttaaaa gcagagagtg atgaagcaca tgaaggttct atggttcatt ccgacaatgg
     3061 aagagacaaa gtccaccatt cccagccacc tttctgctct tctggtgaca gcgaaagtga
     3121 ctctgacagt gcagagaatg ggtggggcaa tggctccaac tcatcagaag acactgacac
     3181 tcacaaaggc cccaaacaca agctgacata caatagaaag gacctgttgg aagttcctga
     3241 gataaaagct gaagatgaca agtttatccc ttgtgagaac aggtgtgatt ctgacacaga
     3301 tgggagagac ccacagaact ctcatatgga acccttggtt gtgaaagccc agccttcctt
     3361 cccagaggtt gaagagggtg agagcctggc cacagtaaca gaagagcctg ctgaggtgga
     3421 gaaagcaaag gggaacctga gtttgctgga acaggctatt gctctacagg ctgaacgagg
     3481 ttctgtcttc catcacactt ataaggagct ggaccgattt ttcctggacc acctggcaag
     3541 ggaacggagg caacctagag ttactgatgc aaatggaaga caaatcttta ctaataaaca
     3601 ttcaccaaga cctgaaagga gggaggccaa gtgccccatt cctggctgtg atggcacggg
     3661 gcacgtaaca gggctctacc ctcaccaccg cagcctttct gggtgccccc acaaagtgcg
     3721 agttcctctg gaaattctcg ccatgcatga gaacgtgctc aagtgtccca cgccaggatg
     3781 cacaggaagg ggacatgtga acagcaatcg caacactcac agaagtcttt ctggttgtcc
     3841 gattgctgca gctgaaaagc tggcaatgac ccaggacaaa agtcaacttg attcttctca
     3901 gacggggcag tgtcctgagc aggcacacag ggtgaatttg gtgaagcaaa ttgaatttaa
     3961 tttccgctca catgccatca cttctccaag agcctctgca tcaaaagaac aagagaagtt
     4021 tgggaaagta ccgtttgatt atgccagttt tgatgcacaa gtatttggga aacgtcctct
     4081 cctacaaaca gggcaaggac aaaaagcacc accatttcct gaatcaaagc atttttcaaa
     4141 tccagtgaaa tttcctaatg gactgcctag tgctggcgcc cacacacaga gcacagtccg
     4201 tgccagctct tatggccatg gtcagtacag tgaagacacc cacatagcag cagctgctgc
     4261 catcctgaac ctttccaccc gctgcaggga agccacagac atcctctcca acaaaccaca
     4321 aagcctgcgt gccaagggag ctgagataga agtagatgaa aacggcacat tggacttaag
     4381 catgaaaaaa aatcgaatcc acgacaagtc tataccccca acttcctcac ctactacaat
     4441 tacaacccca tcctcatccc cattcaacgc aagcagcctt ctggtcaatg ctgccttcta
     4501 tcaggccctc tctgatcaag aaggctggaa tgtgcccatc aactatagca aatcccatgg
     4561 gaagacagag gaggagaaag agaaagatcc tgtgaacttc ctagaaaatt tagaggaaaa
     4621 aaagtttgct ggagaggcct ctatcccaag cccaaagccc aagctgcata caagagatct
     4681 caagaaagaa ttaatcacct gtccaacacc aggatgtgat ggaagcggcc atgtcacagg
     4741 caactatgca tctcaccgca gtgtctctgg atgtcctcta gcagataaga ctctgaagtc
     4801 tctcatggct gccaactctc aagagcttaa gtgtccaacc ccaggttgtg atggttctgg
     4861 tcatgtgact ggaaactatg cctcccaccg aagcttgtct ggttgccctc gtgcaaggaa
     4921 aggtggcatc aaaatgaccc caacaaaaga agaaaaagaa gactctgaac ttagatgccc
     4981 tgtaataggg tgtgacggcc aaggccacat atcaggtaaa tacacatcac accgcacagc
     5041 ttctggctgt cctctggctg ccaaaagaca gaaagagaat cccctcaatg gggcacctct
     5101 ctcctggaaa ctgaacaagc aagagcttcc tcactgtcct ctgccaggat gcaatggtct
     5161 gggtcatgta aacaacgttt ttgtcaccca cagaagctta tctggatgcc ctcttaatgc
     5221 acaagctatc aaaaaagtca aggtctctga ggaactaatg actatcaagc tcaaagcaac
     5281 tgggggtatt gatggtgatg aagaaattag gcatctggat gaagaaatca aggaactgaa
     5341 tgaatccaac cttaaaattg aagcagatat gatgaaactt cagacccaga taacatctat
     5401 ggagagcaac ttgaagacca tagaggagga gaacaagctt atagaacaga gcaatgagag
     5461 tctgctgaag gagctggcag ggcttagcca ggctctcatc tccagccttg ctgacatcca
     5521 acttccacag atggggccta tcaatgagca gaattttgaa gcatatgtaa atactctcac
     5581 agacatgtac agcaatctgg aacaggacta ttccccagaa tgcaaagctc tactggaaag
     5641 catcaagcag gcggtgaagg gcatccatgt gtaggaattc taccgggtag gggaggcgct
     5701 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac
     5761 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt
     5821 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc
     5881 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt
     5941 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata
     6001 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg
     6061 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt
     6121 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
     6181 ttcgacctgc agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc
     6241 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     6301 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     6361 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     6421 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     6481 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     6541 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     6601 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     6661 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     6721 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     6781 atgacccgca agcccggtgc ctgacggggc gcgtctggaa caatcaacct ctggattaca
     6841 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     6901 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     6961 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     7021 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     7081 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     7141 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     7201 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     7261 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     7321 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     7381 gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga
     7441 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct
     7501 agaagcacaa gaggaggagg aggtgggttt ttccagtcac acctcaggta cctttaagac
     7561 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg
     7621 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
     7681 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     7741 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     7801 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     7861 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     7921 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
     7981 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     8041 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     8101 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     8161 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     8221 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     8281 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt
     8341 tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     8401 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     8461 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     8521 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     8581 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     8641 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8701 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8761 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8821 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8881 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8941 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     9001 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     9061 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     9121 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     9181 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     9241 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     9301 aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9361 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9421 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9481 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9541 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9601 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9661 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9721 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9781 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9841 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9901 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9961 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    10021 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10081 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10141 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10201 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10261 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10321 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10381 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10441 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10501 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta
    10561 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    10621 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    10681 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta
    10741 ttaccactgc caattaccta gtggtttcat ttactctaaa cctgtgattc ctctgaatta
    10801 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agtttttaaa
    10861 gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
