LOCUS       sequence-109342-       11154 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A lentiviral vector for the expression of mouse Myt1 under 
            doxycyline control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11154)
  AUTHORS   Najm FJ, Lager AM, Zaremba A, Wyatt K, Caprariello AV, Factor DC, 
            Karl RT, Maeda T, Miller RH, Tesar PJ
  TITLE     Transcription factor-mediated reprogramming of fibroblasts to 
            expandable, myelinogenic oligodendrocyte progenitor cells.
  JOURNAL   Nat Biotechnol. 2013 May;31(5):426-33. doi: 10.1038/nbt.2561. Epub 
            2013 Apr 14.
  PUBMED    23584611
REFERENCE   2  (bases 1 to 11154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2561"; 
            journalName: "Nat Biotechnol"; date: "2013-05"; volume: "31"; issue:
            "5"; pages: "426-33"
COMMENT     Sequence Label: pLVX-Tight-Puro_Myt1
FEATURES             Location/Qualifiers
     source          1..11154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2206..2520
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2214..2232
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2250..2268
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2285..2303
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2321..2339
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2357..2375
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2392..2410
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2428..2446
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2493..2517
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4221..4232
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     promoter        5939..6438
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5974..5997)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6371..6390
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6459..7058
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6459..6478)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6955..6975
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7076..7664
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7129..7149)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7547..7558)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7872..8505
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8634..8650)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8634..8650)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8647..8669)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8658..8674
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8682..8688,8689..8706,8707..8712))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8727..8748
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8865..8882)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9036..9621)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9116..9135)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9792..10583,10584..10652))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10415..10434
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10653..10757)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10805..10939
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10842..10861)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10896..10915
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat
     2221 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt
     2281 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
     2341 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
     2401 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
     2461 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2521 tggagaagga tccgcggccg caccatgagc tcagaaagtg atgacaagcg agcccgcaca
     2581 cggtccaaga ccctccgagg acccccagag accacaggtg cagacctcag ttgtcctacc
     2641 ccaggatgta ctggttcagg acatgtgcgg ggcaagtact ccagacatcg aagcttacag
     2701 agttgccctc tggccaagaa gagaaaactg gaggatgctg agactgagca cctggtatcc
     2761 aagaggaagt cacaccctct gaggctggct ctggatgagg gttaccgcat ggacagtgat
     2821 ggcagtgagg atgctgaagt gaaggacgtc tctgtttcag atgaatcaga aggacccctg
     2881 gaggaggctg aggctgagat gtcaggacag gaggagattc atcacccaca gacagctgaa
     2941 ggaaaaagcc tcatcaagcc ccattttgac tccaacccca caagcagccc ttctggcttc
     3001 tccaagagca gctacagtag ctaccagggg atcattgcaa cttccctcct aaatctgggc
     3061 caaattgctg aagaggccct tgtgaaggag gattccgtct cagtagctaa gttgagcccc
     3121 actgttgttc atcagcttca ggatgaggct gcaatggggg tcaacagtga cgagggtgaa
     3181 aaggacctct ttatacagcc agaagatgtg gaggaggtca ttgaagtcac aagtgaacgt
     3241 tcccaggagc catgccccca gtctctgaag gatatggtta gtgaagagtc aagtaagcag
     3301 aaaggagtcc taggtcatga ggaggaggga gaggaggagg aagaagatga agaagaagag
     3361 gatgaggagg aggaggagga gggagaggaa ggagaagaag aagaggagga ggaggaggaa
     3421 gaagaggagg aggaagatga agaggaagag gaggaggagg aggaagcagc tccaaatgtt
     3481 atctttgggg aagacacctc ccatacctct gtccagaagg cgtctcctga gttccgaggc
     3541 ccagagctat ctagtcctaa acctgagtac tcagtcatcg tggaggttcg ctctgatgat
     3601 gacaaggatg aggattcacg ctcccagaag tcagcagtca cagatgaatc agaaatgtat
     3661 gacatgatga cccgtgggaa tttaggcctt ctggaacagg ccattgccct gaaggctgag
     3721 caggtgcgag cggtctgtga gtctggctgt ccacctgctg agcagggcca tctgggccca
     3781 ggagagccag ggaaaatggc aaagcccctg gacgtggtga ggaagagctg ctacagcaaa
     3841 gatccttcca gggtggagaa gcgtgaaatc aagtgtccga cacctggctg tgatggcact
     3901 ggccatgtta ctggattgta ccctcatcac cgcagcctgt ctggctgtcc ccacaaggat
     3961 agaatccctc cagagatctt ggccatgcat gaaaatgtac tgaagtgccc tactcctggc
     4021 tgcacgggcc aaggccatgt gaacagcaac cgcaatactc acagaagttt atctggatgt
     4081 cccattgctg ctgctgaaaa attagccaaa tctcatgaga agcagcaatt gcagacagga
     4141 gatcctccca aaaataactc caattcagat cggatcctca ggcccatgtg ttttgtgaag
     4201 cagcttgagg ttccacctta tgggagctac agacccaatg tggctcccgc cacgcccagg
     4261 gccaacttgg ccaaggagct ggagaagttc tccaaagtca cctttgacta cgcaagtttt
     4321 gatgctcagg tttttggcaa acgtatgctt gccccaaaga ttcagaccag cgaaacctca
     4381 cccaaagcct tccaatgctt cgattactct catgatgctg aggctgcaca catggctgcc
     4441 acagccatcc tgaacctctc cactcgatgt tgggagatgc cagagaacct cagcacaaag
     4501 ccacaggacc tccccagcaa ggctgtggac attgaggtag atgaaaatgg aactctggac
     4561 ttgagcatgc ataaacatcg caagcgggaa aacactttcc ccagcagtag cagctgcagt
     4621 agcagccctg gtgtcaagtc tcctgatgtc tctcaacgtc agagcagtac cagtgccccc
     4681 agcagctcta tgacctcacc ccagtccagc caggcctctc gccaggatga gtgggaccgt
     4741 cctctagact acaccaaacc tagtcggctt cgagaggaag agcctgagga gtcagagcca
     4801 gcagcacact cttttgcttc ttctgaagca gatgatcagg aggtgtcaga agagaacttt
     4861 gaggagcgga agtatccagg ggaagtcacc ctaaccaact ttaagctgaa gtttctttcc
     4921 aaggacataa agaaggaact tctcacctgt cccacccccg gctgtgacgg cagtggccat
     4981 atcaccggga actatgcctc ccatcgcagc ctctctggtt gccctcttgc tgacaagagc
     5041 ctcagaaacc tcatggctgc ccactctgct gacctcaagt gccccacacc tggctgtgat
     5101 ggctctggcc acataacagg gaactatgct tcacaccgga gtttgtcagg ctgcccacgt
     5161 gccaagaaga gtggactcaa ggtggcgcct accaaggatg acaaggagga ccccgagttg
     5221 atgaagtgcc cagttccagg ctgtgtgggg ctcggccaca tcagcggcaa atatgcctct
     5281 cacaggagtg cttctggctg cccactggct gctcggaggc agaaagaggg agctctcaat
     5341 ggttcatctt tctcgtggaa gtcgctgaag aatgagggcc ctacttgccc caccccaggt
     5401 tgcgacggct ctggccatgc caacggcagt ttcctcaccc accgaagttt gtctggctgt
     5461 cccagagcaa cctttgctgg aaagaaagga aaactctcag gggatgagat tctcagccca
     5521 aagttcaaga caagtgatgt gctggagaat gatgaggaga tcaagcagct aaatcaggag
     5581 atacgagact taaatgagtc caattcggaa atggaggctg ccatggtgca gctgcagtct
     5641 cagatctcgt ccatggagaa gaacctgaag aacatcgagg aggagaacaa gctcattgag
     5701 gagcagaatg aggccctgtt tctggaattg tccgggctta gccaagccct catccaaagt
     5761 cttgccaata ttcgccttcc acacatggaa ccaatatgtg aacagaattt tgacgcctat
     5821 gtgaacaccc tcactgacat gtactccaat caggactgct accagaatcc ggagaacaaa
     5881 ggccttctgg aaacgatcaa gcaagctgtg aggggcattc aggtctagga attctaccgg
     5941 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc
     6001 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc
     6061 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg
     6121 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt
     6181 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg
     6241 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt
     6301 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc
     6361 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc
     6421 tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg
     6481 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     6541 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     6601 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     6661 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     6721 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     6781 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     6841 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     6901 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     6961 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     7021 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg gggcgcgtct ggaacaatca
     7081 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     7141 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     7201 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     7261 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     7321 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     7381 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     7441 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     7501 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     7561 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     7621 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc
     7681 agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     7741 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gtttttccag tcacacctca
     7801 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     7861 aaagagggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     7921 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
     7981 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
     8041 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
     8101 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
     8161 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
     8221 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
     8281 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
     8341 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8401 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8461 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     8521 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt
     8581 gacattgcta gcgttttacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     8641 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     8701 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     8761 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     8821 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     8881 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     8941 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     9001 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     9061 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     9121 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9181 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9241 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9301 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     9361 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9421 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     9481 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9541 tgatccggca aacaaaccac cgctggtagc ggtttttttg tttgcaagca gcagattacg
     9601 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     9661 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     9721 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     9781 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     9841 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     9901 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     9961 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    10021 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    10081 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    10141 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    10201 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    10261 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    10321 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    10381 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    10441 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    10501 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    10561 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    10621 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    10681 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    10741 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
    10801 gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    10861 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    10921 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
    10981 caccccatac cctattacca ctgccaatta cctagtggtt tcatttactc taaacctgtg
    11041 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
    11101 tagtagtttt taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
