LOCUS       sequence-109345-         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A lentiviral dual fluorescent protein reporter, HypoxCR, detects 
            hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Le A, Stine ZE, Nguyen C, Afzal J, Sun P, Hamaker M, Siegel NM, Gouw
            AM, Kang BH, Yu SH, Cochran RL, Sailor KA, Song H, Dang CV
  TITLE     Tumorigenicity of hypoxic respiring cancer cells revealed by a 
            hypoxia-cell cycle dual reporter.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Aug 11. pii: 201402012.
  PUBMED    25114222
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2014 Aug 11. pii: 201402012."
COMMENT     Sequence Label: HypoxCR
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(163..180)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    169..393
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    490..605
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 gacgagctgt acaagaagct tagccatggc ttcccgccgg cggtggcggc gcaggatgat
       61 ggcacgctgc ccatgtcttg tgcccaggag agcgggatgg accgtcaccc tgcagcctgt
      121 gcttctgcta ggatcaatgt gatctagaag ctcgctgatc agcctcgact gtgccttcta
      181 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
      241 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
      301 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
      361 gcaggcatgc tggggatggc ccgggctcta tggcttctga ggcggaaaga accagctggg
      421 gctctagggg gtatccccac gcgccctgat cgataaactg gatctctgct gtccctgtaa
      481 taaacccgaa aattttgaat ttttgtaatt tgtttttgta attctttagt ttgtatgtct
      541 gttgctatta tgtctactat tctttcccct gcactgtacc ccccaatccc cccttttctt
      601 ttaaaggcga taccgtcgag atccgttcac taatcgaatg gatctgtctc tgtctctctc
      661 tccaccttct tc
//
