LOCUS       sequence-109346-         550 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A lentiviral dual fluorescent protein reporter, HypoxCR, detects 
            hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Le A, Stine ZE, Nguyen C, Afzal J, Sun P, Hamaker M, Siegel NM, Gouw
            AM, Kang BH, Yu SH, Cochran RL, Sailor KA, Song H, Dang CV
  TITLE     Tumorigenicity of hypoxic respiring cancer cells revealed by a 
            hypoxia-cell cycle dual reporter.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Aug 11. pii: 201402012.
  PUBMED    25114222
REFERENCE   2  (bases 1 to 550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2014 Aug 11. pii: 201402012."
COMMENT     Sequence Label: HypoxCR
FEATURES             Location/Qualifiers
     source          1..550
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(32..51)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     528..548
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct
       61 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
      121 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
      181 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
      241 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
      301 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
      361 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
      421 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
      481 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
      541 ctacgagcgg
//
