LOCUS       sequence-109347-         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CrBlue is a synthetic chimera comprising the blue opsin backbone 
            with the intracellular loops and the C terminus of the jellyfish 
            opsin, created to introduce spectral selectivity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Karunarathne WK, Giri L, Kalyanaraman V, Gautam N
  TITLE     Optically triggering spatiotemporally confined GPCR activity in a 
            cell and programming neurite initiation and extension.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1565-74. doi: 
            10.1073/pnas.1220697110. Epub 2013 Mar 11.
  PUBMED    23479634
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220697110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-04-23- 23"; volume: "110"; issue: "17"; pages: 
            "E1565-74"
COMMENT     Sequence Label: CrBlue ---- pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             288..305
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cggccgcagc tttcagcagg agccacctgg gaggtctcgt taggttcaac agccgtactg
       61 gaaggagcaa tggcattacc accgacaatt cttccacaaa caccacgtaa tacagctctt
      121 tggaactgct tgttcatgaa gcagtagatg atagggttgt aaatcgatgc ggacttggag
      181 aagaacgaag ggattgtcac cagtcgcaga tctaaaccgt ggttcctgtt gttcaccatg
      241 tacatggcga aagcagcgta gggtacgtag cacacgcaga aggatcccac catcaccacc
      301 accattcttg tgtgtcgctt ttcggccttg atattcttta gtgcagcctc ggattctgaa
      361 ccaaacaact gtgctgcacg acctcgcata ttcttcatct ctccttgaac caaggtgtag
      421 gagaagcaga tcaggctcag aggcacgatg aagcagaaga tgaacaggaa ccaggtgtag
      481 ctctccgatc ggtactttgt acccacggtg taccagtcag ggccacagct gcactgaagg
      541 ccttccggaa tgaagcgaga ccaaccgaag aagggaggga tcgatacacc gataccgatt
      601 gtccaggtag ccagtactac ggttaacgca tttctcattg atccgtgaat cgcagttgcc
      661 acgaaaggtc tgcatatgac gatgtagcgt tcgaaggcca ggaaggccag agaccacccg
      721 gtgaccagac cagccacggt acctaggaaa ccctccagag cacacacgtg acgtccgaac
      781 acgaagtaac cgttgcagct agccacgaac aca
//
