LOCUS       sequence-109354-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Blue opsin tagged with mcherry on the C terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Karunarathne WK, Giri L, Kalyanaraman V, Gautam N
  TITLE     Optically triggering spatiotemporally confined GPCR activity in a 
            cell and programming neurite initiation and extension.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1565-74. doi: 
            10.1073/pnas.1220697110. Epub 2013 Mar 11.
  PUBMED    23479634
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220697110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-04-23- 23"; volume: "110"; issue: "17"; pages: 
            "E1565-74"
COMMENT     Sequence Label: Blue opsin mcherry ----  pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..61
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        42..60
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tggctactag agaacccact gccttactgg cttatcgaaa ttaatacgac tcactatagg
       61 gagacccaag ctggctagcg tttaaactta agcttggtac cgagctcgga tccactagtc
      121 cagtgtggtg gaattcatgc gcaagatgtc cgaggaggag ttctacctgt tcaagaacat
      181 ctcgagcgtc ggcccttggg acgggcccca gtaccacatc gctcctgtct gggctttcta
      241 cctccaggct gctttcatgg ggaccgtgtt cctcatcggt ttccctctga atgccatggt
      301 gctcgtggcc accctgcgtt acaagaagct gcgacagcct ttaaactaca tcctggtgaa
      361 cgtgtccttc ggtggattcc tgctgtgtat cttctccgtg ttccctgtgt tcgtggctag
      421 ctgcaacggt tacttcgtgt tcggacgtca cgtgtgtgct ctggagggtt tcctaggtac
      481 cgtggctggt ctggtcaccg ggtggtctct ggccttcctg gccttcgaac gctacatcgt
      541 catatgcaag cctttcggta acttcaggtt ctcctccaag cacgcgttaa ccgtagtact
      601 ggctacctgg acaatcggta tcggtgtatc gatccctccc ttcttcggtt ggtctcgctt
      661 cattccggaa ggccttcagt gcagctgtgg ccctgactgg tacaccgtgg gtacaaagta
      721 ccgatcggag agctacacct ggttcctgtt catcttctgc ttcatcgtgc ctctgagcct
      781 gatctgcttc tcctacaccc aactgctgcg agctctgaag
//
