LOCUS       sequence-109357-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Blue opsin tagged with mcherry on the C terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Karunarathne WK, Giri L, Kalyanaraman V, Gautam N
  TITLE     Optically triggering spatiotemporally confined GPCR activity in a 
            cell and programming neurite initiation and extension.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1565-74. doi: 
            10.1073/pnas.1220697110. Epub 2013 Mar 11.
  PUBMED    23479634
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220697110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-04-23- 23"; volume: "110"; issue: "17"; pages: 
            "E1565-74"
COMMENT     Sequence Label: Blue opsin mcherry ----  pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(272..291)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     541..559
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      701..710
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             784..795
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 gtaagctcgt ccatgccgcc ggtggagtgg cggccctcgg cgcgttcgta ctgttccacg
       61 atggtgtagt cctcgttgtg ggaggtgatg tccaacttga tgttgacgtt gtaggcgccg
      121 ggcagctgca cgggcttctt ggccttgtag gtggtcttga cctcagcgtc gtagtggccg
      181 ccgtccttca gcttcagcct ctgcttgatc tcgcccttca gggcgccgtc ctcggggtac
      241 atccgctcgg aggaggcctc ccagcccatg gtcttcttct gcattacggg gccgtcggag
      301 gggaagttgg tgccgcgcag cttcaccttg tagatgaact cgccgtcctg cagggaggag
      361 tcctgggtca cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag
      421 ccctcgggga aggacagctt caagtagtcg gggatgtcgg cggggtgctt cacgtaggcc
      481 ttggagccgt acatgaactg aggggacagg atgtcccagg cgaagggcag ggggccaccc
      541 ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc
      601 tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaac
      661 tccttgatga tggccatgtt atcctcctcg cccttgctca ccatggcggc cgcagcagga
      721 gccacctggg aggtctcgtt agggccaacc tgggtggagg acacggtcga cacctcggtc
      781 ttctgggagc tgcaggtgtc gctctcgtcg gtcatagcct taccgcacac catcttcatg
      841 atgcaagctt
//
