LOCUS       sequence-109362-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  G protein Gamma cone(9) subunit tagged with mcherry on the N 
            terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Karunarathne WK, Giri L, Kalyanaraman V, Gautam N
  TITLE     Optically triggering spatiotemporally confined GPCR activity in a 
            cell and programming neurite initiation and extension.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1565-74. doi: 
            10.1073/pnas.1220697110. Epub 2013 Mar 11.
  PUBMED    23479634
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220697110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-04-23- 23"; volume: "110"; issue: "17"; pages: 
            "E1565-74"
COMMENT     Sequence Label: mcherry Gamma 9 ---pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(229..246)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(531..550)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgctggatat ctgcagaatt ctcagcttat tatacaaccg cctttttcct tgaagggatt
       61 cttgtcctca gggatgcctt tgagaaaagg atcgtttcct gcttgggcct ccacgtactc
      121 cttgatttcc tttcccgctt tggaaatcgg aattcttgtg tttttcactt ctttcttcag
      181 ctgctccacc tccatcttca acaggtcctt ctcgctgaga tcctgggcgt gatggtgatg
      241 gtgatgcatg gtacccttgt acagctcgtc catgccgccg gtggagtggc ggccctcggc
      301 gcgttcgtac tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt ccaacttgat
      361 gttgacgttg taggcgccgg gcagctgcac gggcttcttg gccttgtagg tggtcttgac
      421 ctcagcgtcg tagtggccgc cgtccttcag cttcagcctc tgcttgatct cgcccttcag
      481 ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc cagcccatgg tcttcttctg
      541 cattacgggg ccgtcggagg ggaagttggt gccgcgcagc ttcaccttgt agatgaactc
      601 gccgtcctgc agggaggagt cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat
      661 cacgcgctcc cacttgaagc cctcggggaa ggacagcttc aagtagtcgg ggatgtcggc
      721 ggggtgcttc acgtaggcct tggagccgta catgaactga ggggacagga tgtcccaggc
      781 gaagggcagg gggccaccct tggtcacctt cagctt
//
