LOCUS       sequence-109374-        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd generation lentiviral packaging plasmid. Can be used with 2nd or
            3rd generation lentiviral vectors and envelope expressing plasmid 
            (Addgene#12259).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  TITLE     TRONO lab plasmids
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCMVR8.74
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..58
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(594..620)
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             complement(914..958)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gactaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
       61 tttcgtcttc aagaattaat tctcatgttt gacagcttat catcgccact ccagccagcc
      121 ccactcatgt tccccccgca ccacatatgc acatgcacgc atgtacacac aacttgtagt
      181 gctctttcaa aggttttatt cattgcagag gggtggacag ggtagtggtg ggcctagttg
      241 cagtagttct ccagttggta gagagagcag atgctggtgc agcactgatc cacgatgccg
      301 cgcttctgcc gggccacctc cagtgccaag gtctgaaggt cactcgagct tatagcaaaa
      361 tcctttccaa gccctgtctt attcttctag gtatgtggcg aatagctcta caagctcctt
      421 gtactacttc tataacccta tctgtcccct cagctactgc tatggctgtg gcattgagca
      481 agctaacagc actattcttt agctcctgac tccaatattg taggagattc caccaatatt
      541 tgagggcttc ccaccccctg cgtcccagaa gttccacaat cctcgttaca atcaagagta
      601 agtctctcaa gcggtggtag ctgaagaggc acaggctccg cagatcgtcc cagataagtg
      661 ccaaggatcc gttcactaat cgaatggatc tgtctctgtc tctctctcca ccttcttctt
      721 ctattccttc gggcctgtcg ggtcccctcg gggttgggag gtgggtctga aacgataatg
      781 gtgaatatcc ctgcctaact ctattcacta tagaaagtac agcaaaaact attcttaaac
      841 ctaccaagcc tcctactatc attatgaata attttatata ccacagccaa tttgttatgt
      901 taaaccaatt ccacaaactt gcccatttat ctaattccaa taattcttgt tcattctttt
      961 cttgctggtt ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt
     1021 ctctgtccca ctccatccag gtcg
//
