LOCUS       sequence-109378-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; 
            mammalian vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin 
            S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, 
            Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins JJ, 
            Church GM
  TITLE     Highly efficient Cas9-mediated transcriptional programming.
  JOURNAL   Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
  PUBMED    25730490
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312."
COMMENT     Sequence Label: M_ST1n_VPR
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    35..83
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     108..127
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     protein_bind    260..284
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gagtgccagc cctgggaccg aaccccgcgt ttatgaacaa acgacccaac acccgtgcgt
       61 tttattctgt ctttttattg ccgtcatagc gcgggttcct tccggtattg tctccttccg
      121 tgtttcagtt agcctccccc gtttaaactc attactaacc ggtagggatc gaaccctttg
      181 tctgctcgaa gcattaaccc tcactaaagg gaagcggcct tagttattca ggtacctgcg
      241 gccgctctag atgatatcaa ccactttgta caagaaagct gggtttcaaa acagagatgt
      301 gtcgaagatg gacagtcctg tgctgatatg catggcatgc aagaggcact cgtcgttcag
      361 gaaggtatcc agaatctcgt tcaattccgg ggtcaggggt gagtccaggt tcagatcctc
      421 ggtcatggac tcaagtgtgg ttgtcagctc atccagatgg ccccttgggg gcggatggga
      481 aaggtccatt tggccacaga ttgcagcctc ttccttctgg ggaatcacag tatcggccat
      541 ctcccgaagg gctttgacag cctggctcgt ctcttcatcg ggatcctcca acaggtgact
      601 ggcctcggga gtcactgcgg gcgctggatc cagtggctga gggactggtg ccggggtcag
      661 tgacccgact ggctcatgta ctggaccggt tggtgttggt gcgaggctgg cggggagtgg
      721 gcggttggcc catggacttc ctggaggatg aaatggccgg attcgttttg g
//
