LOCUS       sequence-109379-         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAG414 series plasmid with GPD promoter driving expression of 
            dCas9-VPR; cerevisiae vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin 
            S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, 
            Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins JJ, 
            Church GM
  TITLE     Highly efficient Cas9-mediated transcriptional programming.
  JOURNAL   Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
  PUBMED    25730490
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312."
COMMENT     Sequence Label: pAG414GPD-dCas9-VPR
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atatcgaact tccgttgtgt gatcagtttg gcgttcagca gctgccgcca ataatttttc
       61 attttcttga caacttcttc tgaggggacg ttatcactct tccctctagc tttatcggat
      121 cttgtcaaca ctttattatc aatagaatca tctttgagaa aagactgggg cacgatagca
      181 gccacgtcgt agtcggagag ccgattgatg tccagttcct gatccacgta catgtccctg
      241 ccgttctgca ggtagtacag gtagagcttc tcattctgaa gctgggtgtt ttcaactggg
      301 tgttccttaa ggatttggga ccccagttct tttataccct cttcaatcct cttcatcctt
      361 tccctactgt tcttctgtcc cttctgggta gtttggttct ctcgggccat ctcgataacg
      421 atattctcgg gcttatgcct tcccattact ttgacgagtt catccacgac cttaacggtc
      481 tgcagtattc cctttttgat agctgggcta cctgcaagat tagcgatgtg ctcgtgaaga
      541 ctgtccccct ggccagaaac ttgtgctttc tggatgtcct ccttaaaggt gagagagtca
      601 tcatggatca actgcatgaa gttccggttg gcaaatccat cggacttaag aaaatccagg
      661 attgtctttc cactctgctt gtctcggatc ccattgatca gttttcttga cagccgcccc
      721 catcctgtat atcggcgcct cttgagctgt ttcatgactt tgtcgtcgaa gagatgagcg
      781 taagttttca agcgttcttc aatcat
//
