LOCUS       sequence-109390-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses S. pyogenes CRISPR chimeric RNA element with customizable 
            sgRNA from U6 promoter and puromycin resistance from EF-1a promoter.
            3rd generation lentiviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Sanjana NE, Shalem O, Zhang F
  TITLE     Improved vectors and genome-wide libraries for CRISPR screening.
  JOURNAL   Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047.
  PUBMED    25075903
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2014-08"; volume: "11"; issue: 
            "8"; pages: "783-4"
COMMENT     Sequence Label: lentiGuide-Puro
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(527..738)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 ccgcccaggg cggggcgata cacggcggcg cgaggccagg caccagagca ggccggccag
       61 cttgagacta cccccgtccg attctcggtg gccgcgctcg caggccccgc ctcgccgaac
      121 atgtgcgctg ggacgcacgg gccccgtcgc cgcccgcggc cccaaaaacc gaaataccag
      181 tgtgcagatc ttggcccgca tttacaagac tatcttgcca gaaaaaaagc gtcgcagcag
      241 gtcatcaaaa attttaaatg gctagagact tatcgaaagc agcgagacag gcgcgaaggt
      301 gccaccagat tcgcacgcgg cggccccagc gcccaggcca ggcctcaact caagcacgag
      361 gcgaaggggc tccttaagcg caaggcctcg aactctccca cccacttcca acccgaagct
      421 cgggatcaag aatcacgtac tgcagccagg tggaagtaat tcaaggcacg caagggccat
      481 aacccgtaaa gaggccaggc ccgcgggaac cacacacggc acttacctgt gttctggcgg
      541 caaacccgtt gcgaaaaaga acgttcacgg cgactactgc acttatatac ggttctcccc
      601 caccctcggg aaaaaggcgg agccagtaca cgacatcact ttcccagttt accccgcgcc
      661 accttctcta ggcaccggat caattgccga cccctccccc caacttctcg gggactgtgg
      721 gcgatgtgcg ctctgcccac tgacgggcac cggagccaat tcccactcct ttcaagacct
      781 agaaggtcca ttagctgcaa agattcctct ctgtttaaaa ctttatccat ctttgcaccc
      841 gggccccctc gagccggcgc caaagtggat ctctgctgtc cctgtaataa acccg
//
