LOCUS       sequence-109393-         682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; 
            drosophila vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin 
            S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, 
            Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins JJ, 
            Church GM
  TITLE     Highly efficient Cas9-mediated transcriptional programming.
  JOURNAL   Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
  PUBMED    25730490
REFERENCE   2  (bases 1 to 682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312."
COMMENT     Sequence Label: pAWG-dCas9-VPR
FEATURES             Location/Qualifiers
     source          1..682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agagatgtgt cgaagatgga cagtcctgtg ctgatatgca tggcatgcaa gaggcactcg
       61 tcgttcagga aggtatccag aatctcgttc aattccgggg tcaggggtga gtccaggttc
      121 agatcctcgg tcatggactc aagtgtggtt gtcagctcat ccagatggcc ccttgggggc
      181 ggatgggaaa ggtccatttg gccacagatt gcagcctctt ccttctgggg aatcacagta
      241 tcggccatct cccgaagggc tttgacagcc tggctcgtct cttcatcggg atcctccaac
      301 aggtgactgg cctcgggagt cactgcgggc gctggatcca gtggctgagg gactggtgcc
      361 ggggtcagtg acccgactgg ctcatgtact ggaccggttg gtgttggtgc gaggctggcg
      421 gggagtgggc ggttggccca tggacttcct ggaggatgaa atggccggat tcgttttggc
      481 tggcacacct cgcggccctc aaacacgtca ctaatagcgg agccggcctc aggcttcggc
      541 aaaaacatcc cttccctgga atcccgagat cccgatccgc tacccagcag tgcggagaag
      601 tccatgtcgg cgatagagct gaagtcctcg tcgccagaca gcagtccatt aggcaggcct
      661 ggtgctccca gaggggcagg ag
//
