LOCUS       sequence-109410-        1068 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of MBP-MEI-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Wang JT, Smith J, Chen BC, Schmidt H, Rasoloson D, Paix A, Lambrus 
            BG, Calidas D, Betzig E, Seydoux G
  TITLE     Regulation of RNA granule dynamics by phosphorylation of 
            serine-rich, intrinsically disordered proteins in C. elegans.
  JOURNAL   Elife. 2014 Dec 23;4. doi: 10.7554/eLife.04591.
  PUBMED    25535836
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-12-23"; volume: "4. doi"; pages: " 10.7554/eLife.04591"
COMMENT     Sequence Label: pJW4.18
FEATURES             Location/Qualifiers
     source          1..1068
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..41
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    45..69
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 acaacaataa caataacaac aacctcggga tcgagggaag gatcacaagt ttgtacaaaa
       61 aagcaggctt aatgaatggg gatgtgcagt cagtcattcg cggatatctg gagcgagccc
      121 aagtcgcgaa aacaatgtcc gatgccggac gatggaatga ggctggtgat cttcttcgtc
      181 agctcatgac agatgtcaaa agctgtaaaa tttcggcaag caacagagac gagcatgacg
      241 caagaaatac attcctacga gccctagagg ctaatttgaa gcttgtccaa caaaatgtgc
      301 gcgatgaaga tgacctacac gaggcgatga cgcgacaaag tggaagtcca gagccacctg
      361 cagacccaga tgtttggtcc aaaccatcgc caccgttacc atcctcatcg aaatttggag
      421 caacaaagaa aggagttgga gcagcaggtc cacgtcccag agaaatatca aagtcaacgt
      481 cgtcgatgag cacgaatccg gctgatgtga agcctgcgaa tccaacacaa ggaatactgc
      541 ctcaaaatag tgctggagat tcattcgatg catcggctta tgatgcgtac attgttcaag
      601 cggtgcgtgg tacaatggct acaaatacgg aaaatacaat gtctttagat gatataatcg
      661 gaatgcacga cgtgaagcaa gttctgcacg aagccgtcac tcttccactc ttggtgccag
      721 aattcttcca gggtctccgt tcaccatgga aagcaatggt tctcgctgga ccacctggaa
      781 ctggaaagac tcttatcgca cgtgcgattg cttcagagtc tagttcaact ttttttaccg
      841 tttcctcgac tgatctgtcc agcaagtggc gtggtgattc cgagaagatt gttcgtctgt
      901 tattcgaact tgcacggttc tatgctccat ccataatctt catcgatgag attgacacac
      961 ttggtggaca acgtggaaat tctggagaac acgaagcgag tcggcgtgtt aaatccgagt
     1021 ttctggtaca aatggacggg tcacagaata aattcgattc gcgacgtg
//
