LOCUS       sequence-109411-         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral, doxycycline-inducible system to express transgene of 
            interest and EGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Shuen WH, Kan R, Yu Z, Lung HL, Lung ML
  TITLE     Novel lentiviral-inducible transgene expression systems and 
            versatile single-plasmid reporters for in vitro and in vivo cancer 
            biology studies.
  JOURNAL   Cancer Gene Ther. 2015 Feb 27. doi: 10.1038/cgt.2015.9.
  PUBMED    25721206
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Gene
            Ther. 2015 Feb 27. doi: 10.1038/cgt.2015.9."
COMMENT     Sequence Label: pTet-IRES-EGFP
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    join(60..78,79..101,102..120,121..143,144..162,163..185,
                     186..204,205..227,228..246,247..269,270..288,289..311,
                     312..330)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        363..401
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     398..422
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    593..710
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 atctcgacgg tatcgatcac gagactagcc tcgaccatcg ctagagtcga gtttaccact
       61 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
      121 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
      181 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
      241 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
      301 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg tacccgggtc
      361 gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg aaccgtcaga
      421 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      481 gcctccgcgg ccccgaattg tgagatctca accggtttag gcggccgctt ctgctagcta
      541 attcgagctc ggtacccggg gatcctctag aatctcgacg gtatcggtta acttttaaaa
      601 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
      661 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt atcgatcacg
      721 agactagcct cgaggtttaa actacgggct gcaggaattc cg
//
