LOCUS       sequence-109413-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral, doxycycline-inducible system to express transgene of 
            interest and EGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Shuen WH, Kan R, Yu Z, Lung HL, Lung ML
  TITLE     Novel lentiviral-inducible transgene expression systems and 
            versatile single-plasmid reporters for in vitro and in vivo cancer 
            biology studies.
  JOURNAL   Cancer Gene Ther. 2015 Feb 27. doi: 10.1038/cgt.2015.9.
  PUBMED    25721206
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Gene
            Ther. 2015 Feb 27. doi: 10.1038/cgt.2015.9."
COMMENT     Sequence Label: pTet-IRES-EGFP
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..47
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     32..48
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(169..190)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     518..537
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gtaatccaga ggttgattat cggaattccc tcgaggtcga cggtatcgcg ataagcttga
       61 tatcgaattc gaagcttgag ctcgactagc tagctagcta ctagctagtc gagatctgag
      121 tccggacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag
      181 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag
      241 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
      301 gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat
      361 gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt tgtactccag
      421 cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt
      481 caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt
      541 gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg
      601 ctgcttcatg tggtcggggt agcggctgaa gcactgcacg ccgtaggtca gggtggtcac
      661 gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt
      721 gccgta
//
