LOCUS       sequence-109416-         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression and purification of USP47..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Parsons JL, Dianova II, Khoronenkova SV, Edelmann MJ, Kessler BM, 
            Dianov GL
  TITLE     USP47 is a deubiquitylating enzyme that regulates base excision 
            repair by controlling steady-state levels of DNA polymerase beta.
  JOURNAL   Mol Cell. 2011 Mar 4;41(5):609-15. doi: 
            10.1016/j.molcel.2011.02.016.
  PUBMED    21362556
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2011.02"; journalName: "Mol Cell"; date: 
            "2011-03-4- 4"; volume: "41"; issue: "5"; pages: "609-15"
COMMENT     Sequence Label: pDEST26-USP47
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             109..126
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    136..160
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc
       61 gatccagcct ccggactcta gcctaggccg cggaccatgg cgtactacca tcaccatcac
      121 catcactcta gatcaacaag tttgtacaaa aaagcagaag ggccgtcaag gcccaccatg
      181 gtgcccggcg aggagaacca actggtcccg aaagaggcac cactggatca taccagtgac
      241 aagtcacttc tcgacgctaa ttttgagcca ggaaagaaga actttctgca tttgacagat
      301 aaagatggtg aacaacctca aatactgctg gaggattcca gtgctgggga agacagtgtt
      361 catgacaggt ttataggtcc gcttccaaga gaaggttctg ggggttctac cagtgattat
      421 gtcagccaaa gctactccta ctcatctatt ttgaataaat cagaaactgg atatgtggga
      481 ctagtaaacc aagcaatgac ttgctatttg aatagccttt tgcaaacact ttttatgact
      541 cctgaattta ggaatgcatt atataagtgg gaatttgaag aatctgaaga agatccagtg
      601 acaagtattc cataccaact tcaaaggctt tttgttttgt tacaaaccag caaaaagaga
      661 gcaattgaaa ccacagatgt tacaaggagc tttggatggg atagtagtga ggcttggcag
      721 cagcatgatg tacaagaact atgcagagtc atgtttgatg c
//
