LOCUS       sequence-109420-         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression and purification of USP47..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Parsons JL, Dianova II, Khoronenkova SV, Edelmann MJ, Kessler BM, 
            Dianov GL
  TITLE     USP47 is a deubiquitylating enzyme that regulates base excision 
            repair by controlling steady-state levels of DNA polymerase beta.
  JOURNAL   Mol Cell. 2011 Mar 4;41(5):609-15. doi: 
            10.1016/j.molcel.2011.02.016.
  PUBMED    21362556
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2011.02"; journalName: "Mol Cell"; date: 
            "2011-03-4- 4"; volume: "41"; issue: "5"; pages: "609-15"
COMMENT     Sequence Label: pDEST26-USP47
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     379..401
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     393..410
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     394..410
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     421..440
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        421..439
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    472..496
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
       61 aaacctctac aaatgtggta tggctgatta tgatcatgaa cagactgtga ggactgaggg
      121 gcctgaaatg agccttggga ctgtgaatct aaaatacaca aacaattaga atcactagct
      181 cctgtgtata atattttcat aaatcatact cagtaagcaa aactctcaag cagcaagcat
      241 atgcagctag tttaacacat tatacactta aaaattttat atttacctta gagctttaaa
      301 tctctgtagg tagtttgtcc aattatgtca caccacagaa gtaaggttcc ttcacaaaga
      361 tcccaagcta gcagttttcc cagtcacgac gttgtaaaac gacggccagt gcctagctta
      421 taatacgact cactataggg agagagctat gacgtcgcat gcacgcgatc aaccactttg
      481 tacaagaaag ctgggcccat gaggcctgag tcttgagtca gatctttatt tggtgctcca
      541 tccagatata tttttagtgc tttctcttta cgaggtgagt atgttacacg atgtccagtc
      601 ttctggagtc gactgctttc ttttttcatc agttcatttc tttgctcatc tgtcaattcc
      661 attaattctt ctgttttatc cctataaaat atgaccgcac catcatcaca gatat
//
