LOCUS       sequence-109424-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Photoactivatable transcription system. Luciferase reporter under the
            control of a tet-inducible promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Nihongaki Y, Yamamoto S, Kawano F, Suzuki H, Sato M
  TITLE     CRISPR-Cas9-based Photoactivatable Transcription System.
  JOURNAL   Chem Biol. 2015 Feb 19;22(2):169-74. doi: 
            10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
  PUBMED    25619936
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2014.12.011"; journalName: "Chem Biol"; date: 
            "2015-02-19- 19"; volume: "22"; issue: "2"; pages: "169-74"
COMMENT     Sequence Label: Tet-inducible Luciferase reporter
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(176..195)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     391..415
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      500..509
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
       61 cctgacctgg ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
      121 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
      181 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
      241 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
      301 caacgccatc agcgacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc
      361 caacttcaag agctagccct atataagcag agctcgttta gtgaaccgtc agatcgcctg
      421 gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg
      481 cggccccggt accgaattcg ccaccatgga agatgccaaa aacattaaga agggcccagc
      541 gccattctac ccactcgaag acgggaccgc cggcgagcag ctgcacaaag ccatgaagcg
      601 ctacgccctg gtgcccggca ccatcgcctt taccgacgca catatcgagg tggacattac
      661 ctacgccgag tacttcgaga tgagcgttcg gctggcagaa gctatgaagc gctatgggct
      721 gaatacaaac catcggatcg tggtgtgcag cgagaatagc ttgcagttct tcatgcccgt
      781 gttgggtgcc ctgttcatcg gtgtggctgt ggccccagct aacgacatct acaacgagcg
      841 cgagctgctg aacagcatgg gcatcagcca gcccaccgtc
//
