LOCUS       sequence-109425-         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Photoactivatable transcription system. mCherry reporter under the 
            control of a tet-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Nihongaki Y, Yamamoto S, Kawano F, Suzuki H, Sato M
  TITLE     CRISPR-Cas9-based Photoactivatable Transcription System.
  JOURNAL   Chem Biol. 2015 Feb 19;22(2):169-74. doi: 
            10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
  PUBMED    25619936
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2014.12.011"; journalName: "Chem Biol"; date: 
            "2015-02-19- 19"; volume: "22"; issue: "2"; pages: "169-74"
COMMENT     Sequence Label: Tet-inducible mCherry reporter
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(173..192)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     388..412
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      497..506
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(648..666)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
       61 gacctggggc gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt
      121 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
      181 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
      241 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
      301 cgccatcagc gacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa
      361 cttcaagagc tagccctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
      421 acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcgg
      481 ccccggtacc gaattcgcca ccatggtgag caagggcgag gaggataaca tggccatcat
      541 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
      601 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
      661 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
      721 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
      781 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
      841 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
      901 ctt
//
