LOCUS       sequence-109426-         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express codon optimized Lin-28 and wild type Nanog. A single 
            plasmid reprogramming system using a mini-intronic plasmid (MIP)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Diecke S, Lu J, Lee J, Termglinchan V, Kooreman NG, Burridge PW, 
            Ebert AD, Churko JM, Sharma A, Kay MA, Wu JC
  TITLE     Novel codon-optimized mini-intronic plasmid for efficient, 
            inexpensive, and xeno-free induction of pluripotency.
  JOURNAL   Sci Rep. 2015 Jan 28;5:8081. doi: 10.1038/srep08081.
  PUBMED    25628230
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-01-28"; volume: "5:8081. doi"; pages: " 
            10.1038/srep08081"
COMMENT     Sequence Label: MIP 339 CoMiP coLin28 2A Nanog IRES eGFP
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(42..107)
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
ORIGIN
        1 gctggatcca cactcatggt gaagggcgaa ttgttcatga gtggacctgg attgctttct
       61 acatccccag ccagtttgag taaatcaaag ttaagagttt gtttgacagg agcgacaatt
      121 ttgagatccc cgttctgggc ctcgggcagc agggtggggc tgtggatctc ttcctcttcc
      181 tctctgaagt aggtgggctt gccctgggca gaaggtccct gctgggcctt cagggggcag
      241 ctggccacca tgtggctgat gctctggcag aagtggcact tcttgggctg gggaggcagc
      301 ttgcactctt tggcgtggtg gtccaggccg ccacagttgt agcaccggtc gcccttgctc
      361 cgccgcttct gcatgctctt gcccttgggc cttctctcgc tgccgatgca gaacacgccg
      421 ccagggccgg tcactctgat ggattccagg cccttggcgg acttcttgaa ggtgaattcc
      481 acggcctcgc cctctttcag ggatctgaag ccttccatgt gcagcttgct ctggtgcacg
      541 aacacatcca caggggggtc cagggccacg ccggctctgg ctgtcatgct caggaagccg
      601 aagcccatcc gcacgttgaa ccacttgcag attccggcgc catgcagcag ctggggttca
      661 tcggcggctc tagcggcatc ttc
//
