LOCUS       sequence-109430-         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53. A single 
            plasmid reprogramming system using a mini-intronic plasmid (MIP)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Diecke S, Lu J, Lee J, Termglinchan V, Kooreman NG, Burridge PW, 
            Ebert AD, Churko JM, Sharma A, Kay MA, Wu JC
  TITLE     Novel codon-optimized mini-intronic plasmid for efficient, 
            inexpensive, and xeno-free induction of pluripotency.
  JOURNAL   Sci Rep. 2015 Jan 28;5:8081. doi: 10.1038/srep08081.
  PUBMED    25628230
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-01-28"; volume: "5:8081. doi"; pages: " 
            10.1038/srep08081"
COMMENT     Sequence Label: MIP 247 CoMiP 4in1 with shRNA p53
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      284..293
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcaggctgag aggtttccag gccgccctga ggcaccagtc ccacgccgac ctgaggtcca
       61 cagtaggcca tgccgccgca aaactcgtag gggggagggc agggagggat gccccacact
      121 tcgctgccag ggcccacgcc tggtccaatt ccaggtccgc ctggagggcc ttgaaaggac
      181 agccaggtcc gagggtccac ccagccaggt tcagggccgc caggtccatc tccccctccg
      241 ccaggaggtg ggctaaaggc gaagtcgctg gccaggtgtc cggccatggt ggcaccggtt
      301 tagctagtca gctagtggac acgcttcagc ttgcggccta aaaaaaaaaa gcaacaccag
      361 gggtcagtgc gggccggggt acctaatgat ttttatcaaa atcattaagt taaggtagat
      421 acacatcttg tcatatgatc aaatggtttc gccaaaaatc aataatcaga caacaagatg
      481 tgcgaactcg atattttaca cgactctctt taccaattct accacaacgt ggtttgcagg
      541 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
      601 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
      661 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
      721 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tag
//
