LOCUS       sequence-109435-         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53. A single 
            plasmid reprogramming system using a mini-intronic plasmid (MIP)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Diecke S, Lu J, Lee J, Termglinchan V, Kooreman NG, Burridge PW, 
            Ebert AD, Churko JM, Sharma A, Kay MA, Wu JC
  TITLE     Novel codon-optimized mini-intronic plasmid for efficient, 
            inexpensive, and xeno-free induction of pluripotency.
  JOURNAL   Sci Rep. 2015 Jan 28;5:8081. doi: 10.1038/srep08081.
  PUBMED    25628230
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-01-28"; volume: "5:8081. doi"; pages: " 
            10.1038/srep08081"
COMMENT     Sequence Label: MIP 247 CoMiP 4in1 with shRNA p53
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(23..43)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(441..452)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    570..681
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
       61 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
      121 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
      181 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
      241 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
      301 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
      361 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
      421 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
      481 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
      541 tttgggccgc ctccccgcct ggactcgagc tgtgccttct agttgccagc catctgttgt
      601 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
      661 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc t
//
