LOCUS       sequence-109437-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Photoactivatable transcription system. Lentiviral expression of 
            ASCL1 sgRNA3. Also contains a CMV-puro-t2A-mCherry expression 
            cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Nihongaki Y, Yamamoto S, Kawano F, Suzuki H, Sato M
  TITLE     CRISPR-Cas9-based Photoactivatable Transcription System.
  JOURNAL   Chem Biol. 2015 Feb 19;22(2):169-74. doi: 
            10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
  PUBMED    25619936
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2014.12.011"; journalName: "Chem Biol"; date: 
            "2015-02-19- 19"; volume: "22"; issue: "2"; pages: "169-74"
COMMENT     Sequence Label: sgRNA3_ASCL1
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        52..127
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        252..555
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        556..759
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     710..730
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(793..812)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 ataagtatcc cttggagaac caccttgttg gatggagagt ttgcaaggag cgttttagag
       61 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
      121 tcggtgcttt ttttctcgag tactaggatc cattaggcgg ccgcgtggat aaccgtatta
      181 ccgccatgca ttagttatta atagtaatca attacggggt cattagttca tagcccatat
      241 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      301 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      361 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
      421 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      481 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      541 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      601 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      661 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      721 ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc
      781 cgctagccca ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
      841 cc
//
