LOCUS       sequence-109443-         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Photoactivatable transcription system. Mammalian expression of 
            sgRNA1 to target pNeurog2-Luciferase reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Nihongaki Y, Yamamoto S, Kawano F, Suzuki H, Sato M
  TITLE     CRISPR-Cas9-based Photoactivatable Transcription System.
  JOURNAL   Chem Biol. 2015 Feb 19;22(2):169-74. doi: 
            10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
  PUBMED    25619936
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2014.12.011"; journalName: "Chem Biol"; date: 
            "2015-02-19- 19"; volume: "22"; issue: "2"; pages: "169-74"
COMMENT     Sequence Label: sgRNA1_pNeurog2-Luciferase reporter
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        217..292
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(326..346)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(326..344)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(365..381)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(365..381)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(378..400)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    389..405
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(413..419,420..437,438..443))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    458..479
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(596..613)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(847..866)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
       61 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
      121 tatcatatgc ttatcgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
      181 aaaggacgaa acaccgtgaa tgatgataat acgatggttt tagagctaga aatagcaagt
      241 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttc
      301 cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
      361 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
      421 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
      481 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
      541 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
      601 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
      661 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
      721 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
      781 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      841 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      901 ccctgccgct taccggatac ctgtccgcc
//
