LOCUS       sequence-109450-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Pore, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-Nup50-N-10
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      92..101
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cctgtgcatt tgaattggtg gccgttgacc gaaccttcca tgaccacctt catacgcata
       61 ttttccttga tcagctcttc gcccttagac accatggtgg cgaccggtgg atcccctccg
      121 gagctggcat cctttttctc cagtaaaatt ttgtgcaact cgtctgcatc ctcgctggtt
      181 tttacccgaa tcaacatggt gactggcatg gtggcattct tctcgtcaat tggtggattt
      241 ggaacacaga cgataagaac gttattcttc cctgttcgcg tacatggcat attgggtgga
      301 atcagaacgt tcagcaatat gttgcctaaa ttggtgtctg cccgcaccaa aagctgtgtc
      361 ttctgatttg ctgtaggttt taaatgcaga gtacctatgc ctttctcttt aaactcattg
      421 tctttcttgt aaaacagttt acactttttg gagtaaaaag cgtcttcttc ttttacttcg
      481 gtaactacta ctttgggtgg ctcatcattc tcttcttcat ctccaccttt gcattcacca
      541 ctgtcacctt ctgcttggcc ctccaatggt ttagtgggaa atggtgaaga gactggttta
      601 ctctgggtag tatctttgcc aaataaactg gagtttccag gggagaaaga aaatccagtc
      661 agggggacag agcttaatga gcccaaaaca gagctatcaa ctttcttgcc gaaattaaat
      721 gaggcacttg tcgctcctag tgatgatggg tccgttttct tttcagatgc cacctccatc
      781 ttcttgtcag gtgtatcttc
//
