LOCUS       sequence-109457-        2528 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-Paxillin-22
FEATURES             Location/Qualifiers
     source          1..2528
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1692..1744
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      1752..1761
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1758..2492
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gatccgctag caccatggac gacctcgatg ccttactggc agacctggaa tccaccacct
       61 cgcatatctc taagcgaccg gtgtttctaa cagaggaaac gccttactcc tacccaactg
      121 gaaaccatac gtaccaggag attgctgtgc caccgccggt gcccccgccg ccttccagtg
      181 aggccctaaa tggcactgtg attgacccct tagaccagtg gcaacccagc gtgtccagat
      241 acggccacca gcaacctcag tcccagtctc cgatatacag ctctagtgcc aaaagctcca
      301 gtgcctctgt tcctagagac gggctcagct ccccttctcc ccgtgccagt gaggaggaac
      361 acgtgtacag cttcccaaac aagcagaagt ctgcagaacc atctcccaca atgaccagca
      421 cctccctggg cagcaacctc tcggaactgg acaggcttct cctggaactg aatgctgttc
      481 aacataatcc ccccagtggc ttctcagcag atgaagtcag cagaagccca tcactgccca
      541 atgtgaccgg acctcactat gtcatcccag agagcagcag ctctgcagga gggaaggctg
      601 caccccctac aaaagaaaaa ccaaagcgga atggtgggcg tgggattgaa gatgtgcgtc
      661 ccagtgtgga gagcctgctg gatgagctgg agagctctgt gccaagccca gtccctgcaa
      721 tcactgtgag ccaaggggag gtgagcagcc cccagcgtgt caacgccagt cagcagcaga
      781 cccgtatctc tgcttcttca gctacacgag aactggatga gctgatggcg tccctctctg
      841 actttaagtt catggcacag gggaaagccg gcggcagcag ctccccacca tccaccaccc
      901 caaagcctgg cagtcagctg gacaccatgc tgggaagtct gcagtctgac ctgaacaaac
      961 tgggggtagc gacggtcgcc aagggtgtgt gcggagcctg caagaagccg attgctgggc
     1021 aggtagttac agccatgggg aagacctggc accctgagca cttcgtctgc acccactgcc
     1081 aggaggagat tggatcacgg aacttctttg agcgggatgg gcagccctac tgtgagaagg
     1141 actatcacaa cctcttctct cctcgctgct actactgcaa tgggccaatc cttgataaag
     1201 tggtgacggc tttggacagg acgtggcacc ctgaacactt tttctgtgcc cagtgtggag
     1261 ttttctttgg acctgaagga tttcatgaga aggatggcaa agcctattgc cgcaaggact
     1321 actttgacat gtttgctccc aagtgtggag gctgtgcccg ggctatcctg gaaaattaca
     1381 tttctgcctt aaacaccctg tggcaccctg agtgctttgt ctgtcgggaa tgtttcacac
     1441 cgttcatcaa cggcagcttc ttcgagcacg acgggcagcc ctactgcgag gtgcattacc
     1501 acgagcgccg cggctcgctg tgctctggct gccagaagcc catcacggga cgctgcatca
     1561 ctgccatggg caagaaattt caccccgaac actttgtctg tgccttctgc ctcaagcagc
     1621 tcaacaaagg aaccttcaaa gaacagaacg acaagcccta ctgtcagaac tgctttctca
     1681 aactcttctg tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg
     1741 atccaccggt cgccaccatg gtgagcaagg gcgaggagct gatcaaggag aacatgcaca
     1801 tgaagctgta catggaaggc accgtgaaca accaccactt caagtgcacc accgaagggg
     1861 agggcaagcc ctacgagggc acccagaccc agaggattaa ggtggtggag ggaggccccc
     1921 tgccgttcgc attcgacatc ctggccacct gctttatgta cgggagcaag accttcatca
     1981 accacaccca gggcatcccc gatttcttta agcagtcctt ccctgagggc ttcacatggg
     2041 agagagtcac cacatacgaa gacgggggcg tgcttaccgt tacccaggac accagcctcc
     2101 aggacggctg cttgatctac aacgtcaagc tcagaggggt gaacttccca tccaacggcc
     2161 ctgtgatgca gaagaaaaca ctcggctggg aggccaccac cgagaccctg taccccgctg
     2221 acggcggcct ggaaggcaga tgcgacatgg ccctgaagct cgtgggcggg ggccacctgc
     2281 actgcaacct gaagaccaca tacagatcca agaaacccgc taagaacctc aagatgcccg
     2341 gcgtctactt tgtggaccgc agactggaaa gaatcaagga ggccgacaat gagacctacg
     2401 tcgagcagca cgaggtggct gtggccagat actgcgacct ccctagcaaa ctggggcaca
     2461 aacttaatgg catggacgag ctgtacaagt aagcggccgc gactctagat cataatcagc
     2521 cataccac
//
