LOCUS       sequence-109459-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express Oct4, Klf4, Sox2, and c-Myc. A single plasmid 
            reprogramming system using a mini-intronic plasmid (MIP)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Diecke S, Lu J, Lee J, Termglinchan V, Kooreman NG, Burridge PW, 
            Ebert AD, Churko JM, Sharma A, Kay MA, Wu JC
  TITLE     Novel codon-optimized mini-intronic plasmid for efficient, 
            inexpensive, and xeno-free induction of pluripotency.
  JOURNAL   Sci Rep. 2015 Jan 28;5:8081. doi: 10.1038/srep08081.
  PUBMED    25628230
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-01-28"; volume: "5:8081. doi"; pages: " 
            10.1038/srep08081"
COMMENT     Sequence Label: MIP 227 CoMiP 4in1 wo shRNA p53
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(159..176)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(410..429)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgcgccagtc ctccgacaga ctgagtcggc cggtggatcc accggtattg ggatcttcac
       61 acagcaggta aggttgcggg ccgggcctgg gccgggtccg ggccgggtat tgcccgccta
      121 atgagcgggc ttttttttct taccccttct tccgcttcct cgctcactga ctcgctgcgc
      181 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
      241 acagaatcag gggataacgc aggaaagaac gtgtgagcaa aaggccagca aaaggccagg
      301 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
      361 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
      421 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
      481 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
      541 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
      601 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
      661 gacttatcgc cactggcagc ggccactggt aacaggatta gcagagcgag gtatgtaggc
      721 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
      781 ggtatctgcg ctctgctg
//
