LOCUS       sequence-109480-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mini-Tn7 vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2005-06"; volume: "2(6)"; pages: "443-8"
COMMENT     Sequence Label: pUC18T-mini-Tn7T-Gm
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(182..229)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(275..361)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(464..558)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(586..602)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(587..603)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 catacgggaa gaagtgatgc actttgatat cgacccaagt accgccacct aacaattcgt
       61 tcaagccgag atcggcttcc cggccgcgga gttgttcggt aaattgtcac aacgccgcgg
      121 ccaattcgat ctagaattcc attgagtaag tttttaagca catcagcttc aaaagcgctc
      181 tgaagttcct atactttcta gagaatagga acttcggaat aggtacttca agatccccaa
      241 ttcgatcgtc cgggccgcaa gctcctagcg gcggatttgt cctactcagg agagcgttca
      301 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      361 gatgcctcaa gctagagagt cattacccca ggcgtttaag ggcaccaata actgccttaa
      421 aaaaattacg ccccgccctg ccactcatcg cagtctagct tggattctca ccaataaaaa
      481 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
      541 gatctatcaa caggagtcca agctcagcta attaagctag cttatcgata ccgtcgacct
      601 cgaaccccac gcccctcttt aatacgacgg gcaatttgca cttcagaaaa tgaagagttt
      661 gctttagcca taacaaaagt ccagtatgct ttttcacagc ataactggac tgatttcagt
      721 ttacaactat tctgtctagt ttaagacttt attgtcatag tttagatcta
//
