LOCUS       sequence-109482-         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mini-Tn7 vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2005-06"; volume: "2(6)"; pages: "443-8"
COMMENT     Sequence Label: pUC18T-mini-Tn7T-Gm
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(190..195,196..212,213..218))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    259..306
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     mobile_element  419..584
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat
       61 gcccgaggca tagactgtac aaaaaaacag tcataacaag ccatgaaaac cgccactgcg
      121 ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac
      181 ttgcattaca gtttacgaac cgaacaggct tatgtcaatt cgatccattg ctgttgacaa
      241 agggaatcag gggatcttga agttcctatt ccgaagttcc tattctctag aaagtatagg
      301 aacttcagag cgcttttgaa gctaattcga tcatgcatga gctcactagt ggatcccccg
      361 ggctgcagga attcctcgag aagcttgggc ccggtacctc gcgaaggcct tgcaggccaa
      421 ccagataagt gaaatctagt tccaaactat tttgtcattt ttaattttcg tattagctta
      481 cgacgctaca cccagttccc atctattttg tcactcttcc ctaaataatc cttaaaaact
      541 ccatttccac ccctcccagt tcccaactat tttgtccgcc cacagcgggg catttttctt
      601 cctgttatgt ttgggcgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
      661 gtattgggcg ctcttccgct tcctcgctca ctgacccgct gcgctcggtc gttcggctgc
      721 ggcgagcggt atcagagctt atcggccag
//
