LOCUS       sequence-109488-         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of C-terminal domain of Nup88.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Lussi YC, Hugi I, Laurell E, Kutay U, Fahrenkrog B
  TITLE     The nucleoporin Nup88 is interacting with nuclear lamin A.
  JOURNAL   Mol Biol Cell. 2011 Apr;22(7):1080-90. doi: 10.1091/mbc.E10-05-0463.
            Epub 2011 Feb 2.
  PUBMED    21289091
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E10-05-0463"; journalName: "Mol Biol Cell"; date: 
            "2011-04"; volume: "22"; issue: "7"; pages: "1080-90"
COMMENT     Sequence Label: pcDNA3.1-Nup88 (551-741)
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             693..722
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             738..755
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctctctggct actgagaacc cactgcttac tggcttatcg aaattaatac gactcactat
       61 agggagaccc aagctggcta gttaagcttg gtaccatgga aaaggacata gcccctcctc
      121 ctgaagaatg ccttcagctc ctcagcagag ccacccaggt gttcagagag cagtacattc
      181 tcaaacagga cttggcaaag gaggagattc agcggagggt caaattatta tgtgaccaaa
      241 aaaagaaaca actagaagat ctcagttatt gtcgagaaga gaggaaaagt ctgcgggaaa
      301 tggctgagcg tttagctgac aaatatgagg aagctaaaga aaaacaagag gatatcatga
      361 acaggatgaa aaaactactt cacagttttc actctgagct cccagttctc tctgatagtg
      421 agcgagacat gaagaaagaa ttacagctga tacctgatca acttcgacat ttgggcaatg
      481 ccatcaaaca ggttactatg aaaaaggatt atcaacagca aaagatggag aaggtgttga
      541 gtcttccaaa acccaccatt attctcagtg cctaccagcg aaagtgcatt cagtccatcc
      601 tgaaagagga gggtgaacat ataagggaaa tggtgaagca aatcaatgat atccgcaatc
      661 atgtaaactt caatctagag ggcccgcggt tcgaacaaaa actcatctca gaagaggatc
      721 tgaatatgca taccggtcat catcaccatc accattgag
//
