LOCUS       sequence-109491-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Photoactivatable transcription system. Mammalian expression of 
            sgRNA1 to target Tet-inducible-luciferase reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Nihongaki Y, Yamamoto S, Kawano F, Suzuki H, Sato M
  TITLE     CRISPR-Cas9-based Photoactivatable Transcription System.
  JOURNAL   Chem Biol. 2015 Feb 19;22(2):169-74. doi: 
            10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
  PUBMED    25619936
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2014.12.011"; journalName: "Chem Biol"; date: 
            "2015-02-19- 19"; volume: "22"; issue: "2"; pages: "169-74"
COMMENT     Sequence Label: sgRNA1_Tet-inducible Luciferase reporter
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..19
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3..19
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     38..58
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        40..58
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_RNA        complement(92..167)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    169..187
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(189..437)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(417..437)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(451..470)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(452..470)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(480..497)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(480..496)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(489..511)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(725..744)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
       61 acaaaagctg gagctccacc gcggaaaaaa agcaccgact cggtgccact ttttcaagtt
      121 gataacggac tagccttatt ttaacttgct atttctagct ctaaaacctc cctatcagtg
      181 atagagacgg tgtttcgtcc tttccacaag atatataaag ccaagaaatc gaaatacttt
      241 caagttacga taagcatatg atagtccatt ttaaaacata attttaaaac tgcaaactac
      301 ccaagaaatt attactttct acgtcacgta ttttgtacta atatctttgt gtttacagtc
      361 aaattaattc caattatctc tctaacagcc ttgtatcgta tatgcaaata tgaaggaatc
      421 atgggaaata ggccctcggt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
      481 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      541 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
      601 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
      661 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
      721 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
      781 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
      841 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttt
//
