LOCUS       sequence-109498-       14194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mTagBFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14194)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pVSVdG-4BFP2
FEATURES             Location/Qualifiers
     source          1..14194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(90..109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     300..321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        658..1037
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1038..1241
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1192..1212
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1286..1305
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1286..1304
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4462..5163
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(11866..11884)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      11880..11927
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(11950..11966)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11950..11966)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(12003..12023)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12003..12021)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12042..12058)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12042..12058)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12055..12077)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12066..12082
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12090..12096,12097..12114,12115..12120))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12135..12156
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12273..12290)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12444..13032)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12524..12543)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13203..13994,13995..14063))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13826..13845
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14064..14168)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
       61 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      301 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      361 ataagggatt ttgccgattt cggcctattg gttaaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgataag cttgatatcg aattcctgca gcgcgttgac
      661 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      721 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      781 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      841 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      901 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      961 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     1021 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     1081 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     1141 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     1201 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     1261 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
     1321 agattaagcg tctgatgagt ccgtgaggac gaaacccggc gtaccgggtc acgaagacaa
     1381 acaaaccatt attatcatta aaaggctcag gagaaacttt aacagtaatc aaaatgtctg
     1441 ttacagtcaa gagaatcatt gacaacacag tcatagttcc aaaacttcct gcaaatgagg
     1501 atccagtgga atacccggca gattacttca gaaaatcaaa ggagattcct ctttacatca
     1561 atactacaaa aagtttgtca gatctaagag gatatgtcta ccaaggcctc aaatccggaa
     1621 atgtatcaat catacatgtc aacagctact tgtatggagc attaaaggac atccggggta
     1681 agttggataa agattggtca agtttcggaa taaacatcgg gaaagcaggg gatacaatcg
     1741 gaatatttga ccttgtatcc ttgaaagccc tggacggcgt acttccagat ggagtatcgg
     1801 atgcttccag aaccagcgca gatgacaaat ggttgccttt gtatctactt ggcttataca
     1861 gagtgggcag aacacaaatg cctgaataca gaaaaaagct catggatggg ctgacaaatc
     1921 aatgcaaaat gatcaatgaa cagtttgaac ctcttgtgcc agaaggtcgt gacatttttg
     1981 atgtgtgggg aaatgacagt aattacacaa aaattgtcgc tgcagtggac atgttcttcc
     2041 acatgttcaa aaaacatgaa tgtgcctcgt tcagatacgg aactattgtt tccagattca
     2101 aagattgtgc tgcattggca acatttggac acctctgcaa aataaccgga atgtctacag
     2161 aagatgtaac gacctggatc ttgaaccgag aagttgcaga tgaaatggtc caaatgatgc
     2221 ttccaggcca agaaattgac aaggccgatt catacatgcc ttatttgatc gactttggat
     2281 tgtcttctaa gtctccatat tcttccgtca aaaaccctgc cttccacttc tgggggcaat
     2341 tgacagctct tctgctcaga tccaccagag caaggaatgc ccgacagcct gatgacattg
     2401 agtatacatc tcttactaca gcaggtttgt tgtacgctta tgcagtagga tcctctgccg
     2461 acttggcaca acagttttgt gttggagata acaaatacac tccagatgat agtaccggag
     2521 gattgacgac taatgcaccg ccacaaggca gagatgtggt cgaatggctc ggatggtttg
     2581 aagatcaaaa cagaaaaccg actcctgata tgatgcagta tgcgaaaaga gcagtcatgt
     2641 cactgcaagg cctaagagag aagacaattg gcaagtatgc taagtcagaa tttgacaaat
     2701 gaccctataa ttctcagatc acctattata tattatgcta catatgaaaa aaactaacag
     2761 atatcatgga taatctcaca aaagttcgtg agtatctcaa gtcctactct cgtctagatc
     2821 aggcggtagg agagatagat gagatcgaag cacaacgagc tgaaaagtcc aattatgagt
     2881 tgttccaaga ggacggagtg gaagagcata ctaggccctc ttattttcag gcagcagatg
     2941 attctgacac agaatctgaa ccagaaattg aagacaatca aggcttgtat gtaccagatc
     3001 cggaagctga gcaagttgaa ggctttatac aggggccttt agatgactat gcagatgagg
     3061 acgtggatgt tgtattcact tcggactgga aacagcctga gcttgaatcc gacgagcatg
     3121 gaaagacctt acggttgaca ttgccagagg gtttaagtgg agagcagaaa tcccagtggc
     3181 ttttgacgat taaagcagtc gttcaaagtg ccaaacactg gaatctggca gagtgcacat
     3241 ttgaagcatc gggagaaggg gtcatcataa aaaagcgcca gataactccg gatgtatata
     3301 aggtcactcc agtgatgaac acacatccgt accaatcaga agccgtatca gatgtttggt
     3361 ctctctcaaa gacatccatg actttccaac ccaagaaagc aagtcttcag cctctcacca
     3421 tatccttgga tgaattgttc tcatctagag gagaattcat ctctgtcgga ggtaacggac
     3481 gaatgtctca taaagaggcc atcctgctcg gtctgaggta caaaaagttg tacaatcagg
     3541 cgagagtcaa atattctctg tagactatga aaaaaagtaa cagatatcac aatctaagtg
     3601 ttatcccaat ccattcatca tgagttcctt aaagaagatt ctcggtctga aggggaaagg
     3661 taagaaatct aagaaattag ggatcgcacc acccccttat gaagaggaca ctagcatgga
     3721 gtatgctccg agcgctccaa ttgacaaatc ctattttgga gttgacgaga tggacaccta
     3781 tgatccgaat caattaagat atgagaaatt cttctttaca gtgaaaatga cggttagatc
     3841 taatcgtccg ttcagaacat actcagatgt ggcagccgct gtatcccatt gggatcacat
     3901 gtacatcgga atggcaggga aacgtccctt ctacaaaatc ttggcttttt tgggttcttc
     3961 taatctaaag gccactccag cggtattggc agatcaaggt caaccagagt atcacgctca
     4021 ctgcgaaggc agggcttatt tgccacatag gatggggaag acccctccca tgctcaatgt
     4081 accagagcac ttcagaagac cattcaatat aggtctttac aagggaacga ttgagctcac
     4141 aatgaccatc tacgatgatg agtcactgga agcagctcct atgatctggg atcatttcaa
     4201 ttcttccaaa ttttctgatt tcagagagaa ggccttaatg tttggcctga ttgtcgagaa
     4261 aaaggcatct ggagcgtggg tcctggattc tatcagccac ttcaaatgag ctagtctagc
     4321 ttccagcttc tgaacaatcc ccggtttact cagtctctcc taattccagc ctttcgaaca
     4381 actaatatcc tgtcttttct atccctatga aaaaaactaa cagagatcga tctgtttcct
     4441 tgacccggga aagccgccac catgagcgag ctgattaagg agaacatgca catgaagctg
     4501 tacatggagg gcaccgtgga caaccatcac ttcaagtgca catccgaggg cgaaggcaag
     4561 ccctacgagg gcacccagac catgagaatc aaggtggtcg agggcggccc tctccccttc
     4621 gccttcgaca tcctggctac tagcttcctc tacggcagca agaccttcat caaccacacc
     4681 cagggcatcc ccgacttctt caagcagtcc ttccctgagg gcttcacatg ggagagagtc
     4741 accacatacg aagacggggg cgtgctgacc gctacccagg acaccagcct ccaggacggc
     4801 tgcctcatct acaacgtcaa gatcagaggg gtgaacttca catccaacgg ccctgtgatg
     4861 cagaagaaaa cactcggctg ggaggccttc accgagacgc tgtaccccgc tgacggcggc
     4921 ctggaaggca gaaacgacat ggccctgaag ctcgtgggcg ggagccatct gatcgcaaac
     4981 gccaagacca catatagatc caagaaaccc gctaagaacc tcaagatgcc tggcgtctac
     5041 tatgtggact acagactgga aagaatcaag gaggccaaca acgagaccta cgtcgagcag
     5101 cacgaggtgg cagtggccag atactgcgac ctccctagca aactggggca caagcttaat
     5161 taatgaggcg cgcctcgcgg ccgccctgca cacagattct tcatgtttga accaaatcaa
     5221 cttgtgatat catgctcaaa gaggccttaa ttatatttta atttttaatt tttatgaaaa
     5281 aaactaacag caatcatgga agtccacgat tttgagaccg acgagttcaa tgatttcaat
     5341 gaagatgact atgccacaag agaattcctg aatcccgatg agcgcatgac gtacttgaat
     5401 catgctgatt acaatttgaa ttctcctcta attagtgatg atattgacaa tttgatcagg
     5461 aaattcaatt ctcttccgat tccctcgatg tgggatagta agaactggga tggagttctt
     5521 gagatgttaa catcatgtca agccaatccc atctcaacat ctcagatgca taaatggatg
     5581 ggaagttggt taatgtctga taatcatgat gccagtcaag ggtatagttt tttacatgaa
     5641 gtggacaaag aggcagaaat aacatttgac gtggtggaga ccttcatccg cggctggggc
     5701 aacaaaccaa ttgaatacat caaaaaggaa agatggactg actcattcaa aattctcgct
     5761 tatttgtgtc aaaagttttt ggacttacac aagttgacat taatcttaaa tgctgtctct
     5821 gaggtggaat tgctcaactt ggcgaggact ttcaaaggca aagtcagaag aagttctcat
     5881 ggaacgaaca tatgcaggat tagggttccc agcttgggtc ctacttttat ttcagaagga
     5941 tgggcttact tcaagaaact tgatattcta atggaccgaa actttctgtt aatggtcaaa
     6001 gatgtgatta tagggaggat gcaaacggtg ctatccatgg tatgtagaat agacaacctg
     6061 ttctcagagc aagacatctt ctcccttcta aatatctaca gaattggaga taaaattgtg
     6121 gagaggcagg gaaatttttc ttatgacttg attaaaatgg tggaaccgat atgcaacttg
     6181 aagctgatga aattagcaag agaatcaagg cctttagtcc cacaattccc tcattttgaa
     6241 aatcatatca agacttctgt tgatgaaggg gcaaaaattg accgaggtat aagattcctc
     6301 catgatcaga taatgagtgt gaaaacagtg gatctcacac tggtgattta tggatcgttc
     6361 agacattggg gtcatccttt tatagattat tacactggac tagaaaaatt acattcccaa
     6421 gtaaccatga agaaagatat tgatgtgtca tatgcaaaag cacttgcaag tgatttagct
     6481 cggattgttc tatttcaaca gttcaatgat cataaaaagt ggttcgtgaa tggagacttg
     6541 ctccctcatg atcatccctt taaaagtcat gttaaagaaa atacatggcc cacagctgct
     6601 caagttcaag attttggaga taaatggcat gaacttccgc tgattaaatg ttttgaaata
     6661 cccgacttac tagacccatc gataatatac tctgacaaaa gtcattcaat gaataggtca
     6721 gaggtgttga aacatgtccg aatgaatccg aacactccta tccctagtaa aaaggtgttg
     6781 cagactatgt tggacacaaa ggctaccaat tggaaagaat ttcttaaaga gattgatgag
     6841 aagggcttag atgatgatga tctaattatt ggtcttaaag gaaaggagag ggaactgaag
     6901 ttggcaggta gatttttctc cctaatgtct tggaaattgc gagaatactt tgtaattacc
     6961 gaatatttga taaagactca tttcgtccct atgtttaaag gcctgacaat ggcggacgat
     7021 ctaactgcag tcattaaaaa gatgttagat tcctcatccg gccaaggatt gaagtcatat
     7081 gaggcaattt gcatagccaa tcacattgat tacgaaaaat ggaataacca ccaaaggaag
     7141 ttatcaaacg gcccagtgtt ccgagttatg ggccagttct taggttatcc atccttaatc
     7201 gagagaactc atgaattttt tgagaaaagt cttatatact acaatggaag accagacttg
     7261 atgcgtgttc acaacaacac actgatcaat tcaacctccc aacgagtttg ttggcaagga
     7321 caagagggtg gactggaagg tctacggcaa aaaggatgga gtatcctcaa tctactggtt
     7381 attcaaagag aggctaaaat cagaaacact gctgtcaaag tcttggcaca aggtgataat
     7441 caagttattt gcacacagta taaaacgaag aaatcgagaa acgttgtaga attacagggt
     7501 gctctcaatc aaatggtttc taataatgag aaaattatga ctgcaatcaa aatagggaca
     7561 gggaagttag gacttttgat aaatgacgat gagactatgc aatctgcaga ttacttgaat
     7621 tatggaaaaa taccgatttt ccgtggagtg attagagggt tagagaccaa gagatggtca
     7681 cgagtgactt gtgtcaccaa tgaccaaata cccacttgtg ctaatataat gagctcagtt
     7741 tccacaaatg ctctcaccgt agctcatttt gctgagaacc caatcaatgc catgatacag
     7801 tacaattatt ttgggacatt tgctagactc ttgttgatga tgcatgatcc tgctcttcgt
     7861 caatcattgt atgaagttca agataagata ccgggcttgc acagttctac tttcaaatac
     7921 gccatgttgt atttggaccc ttccattgga ggagtgtcgg gcatgtcttt gtccaggttt
     7981 ttgattagag ccttcccaga tcccgtaaca gaaagtctct cattctggag attcatccat
     8041 gtacatgctc gaagtgagca tctgaaggag atgagtgcag tatttggaaa ccccgagata
     8101 gccaagtttc gaataactca catagacaag ctagtagaag atccaacctc tctgaacatc
     8161 gctatgggaa tgagtccagc gaacttgtta aagactgagg ttaaaaaatg cttaatcgaa
     8221 tcaagacaaa ccatcaggaa ccaggtgatt aaggatgcaa ccatatattt gtatcatgaa
     8281 gaggatcggc tcagaagttt cttatggtca ataaatcctc tgttccctag atttttaagt
     8341 gaattcaaat caggcacttt tttgggagtc gcagacgggc tcatcagtct atttcaaaat
     8401 tctcgtacta ttcggaactc ctttaagaaa aagtatcata gggaattgga tgatttgatt
     8461 gtgaggagtg aggtatcctc tttgacacat ttagggaaac ttcatttgag aaggggatca
     8521 tgtaaaatgt ggacatgttc agctactcat gctgacacat taagatacaa atcctggggc
     8581 cgtacagtta ttgggacaac tgtaccccat ccattagaaa tgttgggtcc acaacatcga
     8641 aaagagactc cttgtgcacc atgtaacaca tcagggttca attatgtttc tgtgcattgt
     8701 ccagacggga tccatgacgt ctttagttca cggggaccat tgcctgctta tctagggtct
     8761 aaaacatctg aatctacatc tattttgcag ccttgggaaa gggaaagcaa agtcccactg
     8821 attaaaagag ctacacgtct tagagatgct atctcttggt ttgttgaacc cgactctaaa
     8881 ctagcaatga ctatactttc taacatccac tctttaacag gcgaagaatg gaccaaaagg
     8941 cagcatgggt tcaaaagaac agggtctgcc cttcataggt tttcgacatc tcggatgagc
     9001 catggtgggt tcgcatctca gagcactgca gcattgacca ggttgatggc aactacagac
     9061 accatgaggg atctgggaga tcagaatttc gactttttat tccaagcaac gttgctctat
     9121 gctcaaatta ccaccactgt tgcaagagac ggatggatca ccagttgtac agatcattat
     9181 catattgcct gtaagtcctg tttgagaccc atagaagaga tcaccctgga ctcaagtatg
     9241 gactacacgc ccccagatgt atcccatgtg ctgaagacat ggaggaatgg ggaaggttcg
     9301 tggggacaag agataaaaca gatctatcct ttagaaggga attggaagaa tttagcacct
     9361 gctgagcaat cctatcaagt cggcagatgt ataggttttc tatatggaga cttggcgtat
     9421 agaaaatcta ctcatgccga ggacagttct ctatttcctc tatctataca aggtcgtatt
     9481 agaggtcgag gtttcttaaa agggttgcta gacggattaa tgagagcaag ttgctgccaa
     9541 gtaatacacc ggagaagtct ggctcatttg aagaggccgg ccaacgcagt gtacggaggt
     9601 ttgatttact tgattgataa attgagtgta tcacctccat tcctttctct tactagatca
     9661 ggacctatta gagacgaatt agaaacgatt ccccacaaga tcccaacctc ctatccgaca
     9721 agcaaccgtg atatgggggt gattgtcaga aattacttca aataccaatg ccgtctaatt
     9781 gaaaagggaa aatacagatc acattattca caattatggt tattctcaga tgtcttatcc
     9841 atagacttca ttggaccatt ctctatttcc accaccctct tgcaaatcct atacaagcca
     9901 tttttatctg ggaaagataa gaatgagttg agagagctgg caaatctttc ttcattgcta
     9961 agatcaggag aggggtggga agacatacat gtgaaattct tcaccaagga catattattg
    10021 tgtccagagg aaatcagaca tgcttgcaag ttcgggattg ctaaggataa taataaagac
    10081 atgagctatc ccccttgggg aagggaatcc agagggacaa ttacaacaat ccctgtttat
    10141 tatacgacca ccccttaccc aaagatgcta gagatgcctc caagaatcca aaatcccctg
    10201 ctgtccggaa tcaggttggg ccaattacca actggcgctc attataaaat tcggagtata
    10261 ttacatggaa tgggaatcca ttacagggac ttcttgagtt gtggagacgg ctccggaggg
    10321 atgactgctg cattactacg agaaaatgtg catagcagag gaatattcaa tagtctgtta
    10381 gaattatcag ggtcagtcat gcgaggcgcc tctcctgagc cccccagtgc cctagaaact
    10441 ttaggaggag ataaatcgag atgtgtaaat ggtgaaacat gttgggaata tccatctgac
    10501 ttatgtgacc caaggacttg ggactatttc ctccgactca aagcaggctt ggggcttcaa
    10561 attgatttaa ttgtaatgga tatggaagtt cgggattctt ctactagcct gaaaattgag
    10621 acgaatgtta gaaattatgt gcaccggatt ttggatgagc aaggagtttt aatctacaag
    10681 acttatggaa catatatttg tgagagcgaa aagaatgcag taacaatcct tggtcccatg
    10741 ttcaagacgg tcgacttagt tcaaacagaa tttagtagtt ctcaaacgtc tgaagtatat
    10801 atggtatgta aaggtttgaa gaaattaatc gatgaaccca atcccgattg gtcttccatc
    10861 aatgaatcct ggaaaaacct gtacgcattc cagtcatcag aacaggaatt tgccagagca
    10921 aagaaggtta gtacatactt taccttgaca ggtattccct cccaattcat tcctgatcct
    10981 tttgtaaaca ttgagactat gctacaaata ttcggagtac ccacgggtgt gtctcatgcg
    11041 gctgccttaa aatcatctga tagacctgca gatttattga ccattagcct tttttatatg
    11101 gcgattatat cgtattataa catcaatcat atcagagtag gaccgatacc tccgaacccc
    11161 ccatcagatg gaattgcaca aaatgtgggg atcgctataa ctggtataag cttttggctg
    11221 agtttgatgg agaaagacat tccactatat caacagtgtt tggcagttat ccagcaatca
    11281 tttccgatta ggtgggaggc tatttcagta aaaggaggat acaagcagaa gtggagtact
    11341 agaggtgatg ggctcccaaa agatacccga atttcagact ccttggcccc aatcgggaac
    11401 tggatcagat ctttggaatt ggtccgaaac caagttcgtc taaatccatt caataagatc
    11461 ttgttcaatc agctatgtcg tacagtggat aatcatttga agtggtcaaa tttgcgaaaa
    11521 aacacaggaa tgattgaatg gatcaatggg cgaatttcaa aagaagaccg gtctatactg
    11581 atgttgaaga gtgacctaca tgaggaaaac tcttggagag attaaaaaat caggaggaga
    11641 ctccaaactt taagtatgaa aaaaactttg atccttaaga ccctcttgtg gtttttattt
    11701 ttttatctgg ttttgtggtc ttcgtgggtc ggcatggcat ctccacctcc tcgcggtccg
    11761 acctgggcat ccgaaggagg acgcacgtcc actcggatgg ctaagggagg ggcggggatc
    11821 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac
    11881 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa
    11941 ctatatccgg atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt
    12001 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
    12061 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
    12121 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
    12181 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
    12241 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    12301 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    12361 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    12421 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    12481 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    12541 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    12601 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    12661 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    12721 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    12781 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    12841 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
    12901 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    12961 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    13021 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    13081 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    13141 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    13201 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    13261 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    13321 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    13381 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    13441 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    13501 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    13561 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    13621 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    13681 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    13741 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    13801 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    13861 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    13921 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    13981 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    14041 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    14101 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    14161 gttccgcgca catttccccg aaaagtgcca cctg
//
