LOCUS       sequence-109510-         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal eCitrine Strep-tag II followed by URA3
            selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Rai J, Pemmasani JK, Voronovsky A, Jensen IS, Manavalan A, Nyengaard
            JR, Golas MM, Sander B
  TITLE     Strep-tag II and Twin-Strep Based Cassettes for Protein Tagging by 
            Homologous Recombination and Characterization of Endogenous 
            Macromolecular Assemblies in Saccharomyces cerevisiae.
  JOURNAL   Mol Biotechnol. 2014 Jun 27.
  PUBMED    24969434
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Biotechnol. 2014 Jun 27."
COMMENT     Sequence Label: pGS1412
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(95..292)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 ccgcggccgc ataggccact agtggatctg atatcatcga tgaattcgag ctcgttttcg
       61 acactggatg gcggcgttag tatcgaatcg acagcagtat agcgaccagc attcacatac
      121 gattgacgca tgatattact ttctgcgcac ttaacttcgc atctgggcag atgatgtcga
      181 ggcgaaaaaa aatataaatc acgctaacat ttgattaaaa tagaacaact acaatataaa
      241 aaaactatac aaatgacaag ttcttgaaaa caagaatctt tttattgtca gtactgatta
      301 taattggcca gtctttttca aataagcatt ccaaccagca tctctatacc ttttaccttc
      361 aatatctgga tctcttcctt taccaaacaa tcctctacca acaatgataa tatcagttcc
      421 agtgctaaca acttcatcaa cagttctata ttgttgtcct aatccatcac ctttatcatc
      481 taatccaact ccaggtgtca taataagcca atcaaatcct tcttcttgtc cacccatatc
      541 acgttgggca ataaatccaa taacaaattc cttatcggat ttagcaattt caacagtttt
      601 ttgagaatat tctccatatg ctaatgatcc cactgatgat aattcagcta acatcaataa
      661 ccctcttggc tcttggttgg tggtggtttc tttagctccc tgttttaatc cttcaactac
      721 tccattccca gtgacaccat gagcattagt aatatctgcc caactactaa ttttataaac
      781 tccaccaata tattgtttct tcacggtatt accaatatca gcaaattttc tatcttcaaa
      841 aatcataaat tgatgtttac gtgaaagttc taataatggt tcaatagtgg attcatagga
      901 aaaatcattg attatatcaa tatgagtctt gattaagcat acataaggac ccaatttatc
      961 aattaattca aggaattcct tagtg
//
