LOCUS       sequence-109515-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal eCitrine Strep-tag II followed by URA3
            selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Rai J, Pemmasani JK, Voronovsky A, Jensen IS, Manavalan A, Nyengaard
            JR, Golas MM, Sander B
  TITLE     Strep-tag II and Twin-Strep Based Cassettes for Protein Tagging by 
            Homologous Recombination and Characterization of Endogenous 
            Macromolecular Assemblies in Saccharomyces cerevisiae.
  JOURNAL   Mol Biotechnol. 2014 Jun 27.
  PUBMED    24969434
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Biotechnol. 2014 Jun 27."
COMMENT     Sequence Label: pGS1412
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             54..767
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             777..800
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(886..910)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 aagcttcgta cgctgcaggt cgacggatcg gtgacggtgc tggtttaatt aacatgtcta
       61 aaggtgaaga attattcact ggtgttgtcc caattttggt tgaattagat ggtgatgtta
      121 atggtcacaa attttctgtc tccggtgaag gtgaaggtga tgctacttac ggtaaattga
      181 ccttaaaatt tatttgtact actggtaaat tgccagttcc atggccaacc ttagtcacta
      241 ctttaggtta tggtttgatg tgttttgcta gatacccaga tcatatgaaa caacatgact
      301 ttttcaagtc tgccatgcca gaaggttatg ttcaagaaag aactattttt ttcaaagatg
      361 acggtaacta caagaccaga gctgaagtca agtttgaagg tgatacctta gttaatagaa
      421 tcgaattaaa aggtattgat tttaaagaag atggtaacat tttaggtcac aaattggaat
      481 acaactataa ctctcacaat gtttacatca tggctgacaa acaaaagaat ggtatcaagg
      541 ttaacttcaa aattagacac aacattgaag atggttccgt tcaattagct gaccattatc
      601 aacaaaatac tccaattggt gatggtccag tcttgttacc agacaaccat tacttatcct
      661 atcaatctgc cttatccaaa gatccaaacg aaaagagaga ccacatggtc ttgttagaat
      721 ttgttactgc tgctggtatt acccatggta tggatgaatt gtacaaaggt agtgcttgga
      781 gtcatccaca attcgaaaaa taaggtaccg cgcgcgccac ttctaaataa gcgaatttct
      841 tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt
      901 aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg
      961 tca
//
