LOCUS       sequence-109529-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a binary Agrobacterium vector carrying a modified HSVtk gene as a 
            negative selection marker against ectopic transformants. Uses 
            Gateway cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Khang CH, Park SY, Lee YH, Kang S
  TITLE     A dual selection based, targeted gene replacement tool for 
            Magnaporthe grisea and Fusarium oxysporum.
  JOURNAL   Fungal Genet Biol. 2005 Jun;42(6):483-92. Epub 2005 Apr 25.
  PUBMED    15893252
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2005-06"; volume: "42(6)"; pages: "483-92. Epub
            2005 Apr 25"
COMMENT     Sequence Label: pGKO2-Gateway
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    817..940
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gtctcggaag cccccagcac ctgccagtaa gtcatcggct cgggtacgta gacgatgtcg
       61 tcgcgcgaac ccagggccac cagcagttgc gtggtggtgg ttttccccat cccgtgagga
      121 ccgtctatat aaacccgcag tagcgtgggc attttctgct ccaggcggac ttccgtggct
      181 tcttgctgcc ggcgagggcg caacgccgta cgtcggttgc tatggccgcg agaacgcgca
      241 gcctggtcga acgcagacgc gtgttgatgg caggggtacg aagccatgga tctgaagatt
      301 gggttcctct ttgagagagt taatagactt gaattgagga atggaaatcg ggctgagctc
      361 actgctttat atgtgagaaa aggtgatgag caggtggtaa gaggcagatt tttgtggtcg
      421 gtgctgtgtt ttgttgggtt tgagctaggt ggggtgaggg aggggcaagc taaatagcta
      481 ccagccctga ggacgtcaca gagttttagt agttcttttt ctgccatcgg acatgcagct
      541 cacgcctaga cccaattaca ccctttgcgc cctcccacac atccggccgg ttggatcatt
      601 gtcagatacg gcagagaaat cgcaacctcg gccaagggta gccacgattc gaagccgcga
      661 aattgggggt ttcggaatgt cgtcaagcgg gaaccaattt gagtacccaa ttcgaattca
      721 ccgcggtggc ggccgctcta gaaaataatg attttatttg actgatagtg acctgttcgt
      781 tgcaacaaat tgatgagcaa tgctttttta taatgccaac tttgtacaaa aaagctgaac
      841 gagaagcgta aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga
      901 ctacataata ctgtaaaaca caacatatcc agtcactatg aatcaactac ttagatggta
      961 tta
//
