LOCUS       sequence-109530-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a binary Agrobacterium vector carrying a modified HSVtk gene as a 
            negative selection marker against ectopic transformants. Uses 
            Gateway cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Khang CH, Park SY, Lee YH, Kang S
  TITLE     A dual selection based, targeted gene replacement tool for 
            Magnaporthe grisea and Fusarium oxysporum.
  JOURNAL   Fungal Genet Biol. 2005 Jun;42(6):483-92. Epub 2005 Apr 25.
  PUBMED    15893252
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2005-06"; volume: "42(6)"; pages: "483-92. Epub
            2005 Apr 25"
COMMENT     Sequence Label: pGKO2-Gateway
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(479..710)
                     /gene="mutant version of attP"
                     /label=attP1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
       61 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
      121 gatacagtag aaattacaga aactttatca cgtttagtaa gtatagaggc tgaaaatcca
      181 gatgaagccg aacgacttgt aagagaaaag tataagagtt gtgaaattgt tcttgatgca
      241 gatgattttc aggactatga cactagcgta tatgaatagg tagatgtttt tattttgtca
      301 cacaaaaaag aggctcgcac ctctttttct tatttctttt tatgatttaa tacggcattg
      361 aggacaatag cgagtaggct ggatacgacg attccgtttg agaagaacat ttggaaggct
      421 gtcggtcgac taagttggca gcatcacccg aagaacattt ggaaggctgt cggtcgacta
      481 caggtcacta ataccatcta agtagttgat tcatagtgac tggatatgtt gtgttttaca
      541 gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt
      601 ttacgcttct cgttcagctt ttttgtacaa agttggcatt ataaaaaagc attgctcatc
      661 aatttgttgc aacgaacagg tcactatcag tcaaataaaa tcattatttt ctagagcggc
      721 cgccaccgcg gtgaattcga attgggtact caaattggtt cccgcttgac gacattccga
      781 aacccccaat ttcgcggctt cgaatcgtgg ctacccttgg ccgaggttgc gatttctctg
      841 ccgtatctga caatgatcca accggccgga tgtgtgggag ggcgcaaagg gtgtaattgg
      901 gtctaggcgt gagctgcatg tccgatggca gaaaaagaac tactaaaact ctgtgacgtc
      961 ctcagggctg gtagctattt agcttgcccc tccctcaccc cacctagctc aa
//
