LOCUS       sequence-109532-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Photoactivatable transcription system. Expression of activator 
            probe, which includes the photolyase homology region of CRY2 
            (CRY2PHR) and the transcriptional activator domain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Nihongaki Y, Yamamoto S, Kawano F, Suzuki H, Sato M
  TITLE     CRISPR-Cas9-based Photoactivatable Transcription System.
  JOURNAL   Chem Biol. 2015 Feb 19;22(2):169-74. doi: 
            10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
  PUBMED    25619936
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2014.12.011"; journalName: "Chem Biol"; date: 
            "2015-02-19- 19"; volume: "22"; issue: "2"; pages: "169-74"
COMMENT     Sequence Label: NLSx3-CRY2PHR-p65
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..58)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(119..490)
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
ORIGIN
        1 gatgatgacc ggtacgcgta gaatcgagac cgaggagagg gttagggata ggcttacctt
       61 cgaagggccc tctagactcg agcggccgcc actgtgctgg atatctgcag aattcttagg
      121 agctgatctg actcagcagg gctgagaagt ccatgtccgc aatggaggag aagtcttcat
      181 ctcctgaaag gaggccattg gggagccccg gggcccccag tggagcagga gctgggtcgg
      241 ggggcctctg ggcccctgtc actaggcgag ttatagcctc agggtactcc atcagcatgg
      301 gctcagttgt gtggggggcc acaggtatgc cctggttcag cagctgctga aactcggagt
      361 tgtcgacgga tgccaggtct gtgaacacag ctgggtctgt gctgttgcca agcaaggccc
      421 ccaggtcttc atcatcaaac tgcagctgca gcagggcctc tgacagcgtt ccttccccag
      481 cctgggtggg cttgggggca ggtggggcca cagcctgagg agggcctggg gctaggactg
      541 ggacaggggc tggggcctgg gccagagctg ataccatggc tggagcaggg gcaggggctg
      601 gagcctgggg caggacttgg ggaggggccg gggccaaggc cgaggcctgg ctgatctgcc
      661 cagaaggaaa caccatggtg ggaaactcat catagttgat ggtgctcagg gatgacgtaa
      721 agggataggg ctggggtgct ggcttgggga cagaagctga gctgcgggaa ggcacagcaa
      781 tgcgtcgagg tggaggccgg gggtcggtgg gtccgctgaa aggactcttc ttcatgatgc
      841 tcttgaaggt ctcatatgtc cttttacgtt tctcctcaat ccggtgac
//
