LOCUS       sequence-109536-         682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Myristoylated-FLAG-tagged Akt2, constitutively active.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Lim CY, Bi X, Wu D, Kim JB, Gunning PW, Hong W, Han W
  TITLE     Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated
            GLUT4 exocytosis through cortical actin remodeling.
  JOURNAL   Nat Commun. 2015 Jan 9;6:5951. doi: 10.1038/ncomms6951.
  PUBMED    25575350
REFERENCE   2  (bases 1 to 682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-01-9"; volume: "6:5951. doi"; pages: " 
            10.1038/ncomms6951"
COMMENT     Sequence Label: pLenti-FLAG-Akt2-CA
FEATURES             Location/Qualifiers
     source          1..682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             143..163
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             182..205
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tgtaatcatt tgggtcaata tgtaattttc agtgttagac tagtaaattg tccgctaaat
       61 tctggccgtt tttggctttt ttgttagacg aagcttgggc tgcaggtcga ctctagagga
      121 tcgaattctt cgaagctagg ccatgggttc ctccaaatcc aagcccaagg ctagcgccat
      181 ggactacaag gacgatgacg ataagggaat tgatccaatg aatgaggtgt ctgtcatcaa
      241 agaaggctgg ctccacaagc gtggtgaata catcaagacc tggaggccac ggtacttcct
      301 gctgaagagc gacggctcct tcattgggta caaggagagg cccgaggccc ctgatcagac
      361 tctacccccc ttaaacaact tctccgtagc agaatgccag ctgatgaaga ccgagaggcc
      421 gcgacccaac acctttgtca tacgctgcct gcagtggacc acagtcatcg agaggacctt
      481 ccacgtggat tctccagacg agagggagga gtggatgcgg gccatccaga tggtcgccaa
      541 cagcctcaag cagcgggccc caggcgagga ccccatggac cacaagtgtg gctcccccag
      601 tgactcctcc acgactgagg agatggaagt ggcggtcagc aaggcacggg ctaaagtgac
      661 catgaatgac ttcgactatc tc
//
