LOCUS       sequence-109539-         666 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for F-actin labeling in living cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Lim CY, Bi X, Wu D, Kim JB, Gunning PW, Hong W, Han W
  TITLE     Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated
            GLUT4 exocytosis through cortical actin remodeling.
  JOURNAL   Nat Commun. 2015 Jan 9;6:5951. doi: 10.1038/ncomms6951.
  PUBMED    25575350
REFERENCE   2  (bases 1 to 666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-01-9"; volume: "6:5951. doi"; pages: " 
            10.1038/ncomms6951"
COMMENT     Sequence Label: pLenti-Lifeact-tdTomato
FEATURES             Location/Qualifiers
     source          1..666
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             191..241
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      248..257
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(387..405)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaat
       61 tgtccgctaa attctggccg tttttggctt ttttgttaga cgaagcttgg gctgcaggtc
      121 gactctagag gatcgaattc ttcgaagcta gcgctaccgg actcagatct cgagctcaag
      181 cttcgaattc atgggcgtgg ccgacctgat caagaagttc gagagcatca gcaaggagga
      241 gccggtcgcc accatggtga gcaagggcga ggaggtcatc aaagagttca tgcgcttcaa
      301 ggtgcgcatg gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcgaggg
      361 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc
      421 cttcgcctgg gacatcctgt ccccccagtt catgtacggc tccaaggcgt acgtgaagca
      481 ccccgccgac atccccgatt acaagaagct gtccttcccc gagggcttca agtgggagcg
      541 cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc caggactcct ccctgcagga
      601 cggcacgctg atctacaagg tgaagatgcg cggcaccaac ttcccccccg acggccccgt
      661 aatgca
//
