LOCUS       sequence-109540-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a binary Agrobacterium vector carrying the HSVtk gene as a negative 
            selection marker against ectopic transformants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Khang CH, Park SY, Lee YH, Kang S
  TITLE     A dual selection based, targeted gene replacement tool for 
            Magnaporthe grisea and Fusarium oxysporum.
  JOURNAL   Fungal Genet Biol. 2005 Jun;42(6):483-92. Epub 2005 Apr 25.
  PUBMED    15893252
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2005-06"; volume: "42(6)"; pages: "483-92. Epub
            2005 Apr 25"
COMMENT     Sequence Label: pGKO1
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    51..225
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgggg gatctggatt
       61 ttagtactgg attttggttt taggaattag aaattttatt gatagaagta ttttacaaat
      121 acaaatacat actaagggtt tcttatatgc tcaacacatg agcgaaaccc tataggaacc
      181 ctaattccct tatctgggaa ctactcacac attattatgg agaaactcga aattcgaatt
      241 gggtactcaa attggttccc gcttgacgac attccgaaac ccccaatttc gcggcttcga
      301 atcgtggcta cccttggccg aggttgcgat ttctctgccg tatctgacaa tgatccaacc
      361 ggccggatgt gtgggagggc gcaaagggtg taattgggtc taggcgtgag ctgcatgtcc
      421 gatggcagaa aaagaactac taaaactctg tgacgtcctc agggctggta gctatttagc
      481 ttgcccctcc ctcaccccac ctagctcaaa cccaacaaaa cacagcaccg accacaaaaa
      541 tctgcctctt accacctgct catcaccttt tctcacatat aaagcagtga gctcagcccg
      601 atttccattc ctcaattcaa gtctattaac tctctcaaag aggaacccaa tcttcaaagg
      661 atccatggct tcgtacccct gccatcaaca cgcgtctgcg ttcgaccagg ctgcgcgttc
      721 tcgcggccat agcaaccgac gtacggcgtt gcgccctcgc cggcagcaag aagccacgga
      781 agtccgcctg gagcagaaaa tgcccacgct actgcgggtt tatatagacg gtcctcacgg
      841 gatggggaaa accaccacca cgcaactgct ggtggccctg ggttcgcgcg acgatatcgt
      901 ctacgtaccc
//
