LOCUS       sequence-109547-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a binary Agrobacterium vector carrying a modified HSVtk gene as a 
            negative selection marker against ectopic transformants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Khang CH, Park SY, Lee YH, Kang S
  TITLE     A dual selection based, targeted gene replacement tool for 
            Magnaporthe grisea and Fusarium oxysporum.
  JOURNAL   Fungal Genet Biol. 2005 Jun;42(6):483-92. Epub 2005 Apr 25.
  PUBMED    15893252
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2005-06"; volume: "42(6)"; pages: "483-92. Epub
            2005 Apr 25"
COMMENT     Sequence Label: pGKO2
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     732..748
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     732..748
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(782..798)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(783..799)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(823..842)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(824..842)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggaagccccc agcacctgcc agtaagtcat cggctcgggt acgtagacga tgtcgtcgcg
       61 cgaacccagg gccaccagca gttgcgtggt ggtggttttc cccatcccgt gaggaccgtc
      121 tatataaacc cgcagtagcg tgggcatttt ctgctccagg cggacttccg tggcttcttg
      181 ctgccggcga gggcgcaacg ccgtacgtcg gttgctatgg ccgcgagaac gcgcagcctg
      241 gtcgaacgca gacgcgtgtt gatggcaggg gtacgaagcc atggatctga agattgggtt
      301 cctctttgag agagttaata gacttgaatt gaggaatgga aatcgggctg agctcactgc
      361 tttatatgtg agaaaaggtg atgagcaggt ggtaagaggc agatttttgt ggtcggtgct
      421 gtgttttgtt gggtttgagc taggtggggt gagggagggg caagctaaat agctaccagc
      481 cctgaggacg tcacagagtt ttagtagttc tttttctgcc atcggacatg cagctcacgc
      541 ctagacccaa ttacaccctt tgcgccctcc cacacatccg gccggttgga tcattgtcag
      601 atacggcaga gaaatcgcaa cctcggccaa gggtagccac gattcgaagc cgcgaaattg
      661 ggggtttcgg aatgtcgtca agcgggaacc aatttgagta cccaattcga attcaccgcg
      721 gtggcggccg ctctagaact agtggatccc ccgggctgca ggaattcgat atcaagctta
      781 tcgataccgt cgacctcgag ggggggcccg gtacccaatt cgccctatag tgagtcgtat
      841 tacgcgcgct
//
