LOCUS       sequence-109548-        2387 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid is designed to validate the biophysical model of 
            translational coupling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Tian T, Salis HM
  TITLE     A predictive biophysical model of translational coupling to 
            coordinate and control protein expression in bacterial operons.
  JOURNAL   Nucleic Acids Res. 2015 Aug 18;43(14):7137-51. doi: 
            10.1093/nar/gkv635. Epub 2015 Jun 27.
  PUBMED    26117546
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkv635"; journalName: "Nucleic Acids Res"; date: 
            "2015-08-18- 18"; volume: "43"; issue: "14"; pages: "7137-51"
COMMENT     Sequence Label: pTd-4-15-13-2
FEATURES             Location/Qualifiers
     source          1..2387
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             774..782
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             786..1463
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MKASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIK
                     MRLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     RBS             1477..1485
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      2282..2368
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tctagaataa ccatatacca tttcagcata gatcatatgg tcgacaaggg cgaagaactc
       61 tttaccggcg tcgtcccgat cctggtagag cttgacggcg atgtcaacgg tcataagttt
      121 agcgttagcg gcgaaggcga gggcgacgcg acgtacggta aattaacctt aaaattcatc
      181 tgtacaaccg gtaaattacc ggtaccgtgg cccacgttag ttaccaccct tacctggggc
      241 gtccagtgct tcgcgcgcta tccagaccac atgaagcaac acgatttctt caaatcggct
      301 atgcccgaag gctatgtaca ggagcgcacc atcttcttca aggacgacgg taattataaa
      361 acccgcgcag aagttaagtt cgagggcgat acgctcgtta atcgcatcga actcaaaggt
      421 atcgacttca aagaggacgg caacatttta ggtcacaagt tagagtataa cgcgatttcc
      481 gataacgtct acattaccgc cgataaacaa aagaacggta tcaaagcaaa ctttaagatt
      541 cgtcataaca tcgaggacgg ctcggtacag ctcgccgacc attaccaaca gaatacgccg
      601 atcggcgacg gtccggtcct cctcccggac aatcattatc tcagcaccca gagcgcgctc
      661 tccaaagatc caaacgagaa gcgcgaccac atggtccttc tcgaatttgt aacggcggca
      721 ggcatcaccc tcggtatgga cgaactctat aaggacgtca aaaaatacct ccgtaaggag
      781 gtatcatgaa agcgagctcc gaagacgtta tcaaagaatt tatgcgtttt aaagttcgca
      841 tggagggctc ggttaacggt cacgagttcg aaatcgaagg ggaaggcgaa ggtcgtccgt
      901 acgaaggtac ccagaccgct aaactcaaag ttaccaaagg cggtccctta ccgttcgctt
      961 gggatatttt aagcccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggcgg
     1021 acatcccgga ctaccttaaa ttatccttcc ccgaagggtt taagtgggag cgcgttatga
     1081 acttcgagga cggcggcgtc gttaccgtta cccaggactc ctcccttcaa gacggcgagt
     1141 tcatctacaa agttaaactt cgcggtacca acttcccatc cgatggtccg gtcatgcaga
     1201 agaagaccat gggttgggaa gcttccaccg agcgcatgta tccggaggac ggcgctttaa
     1261 aaggcgaaat caagatgcgc ttaaagttaa aagacggcgg tcactacgac gcagaagtta
     1321 aaacgacgta catggccaag aaaccggttc agttaccggg cgcttacaaa accgacatca
     1381 aactcgacat caccagccac aacgaagact acaccatcgt cgaacagtac gaacgcgcag
     1441 aaggtcgtca cagcaccggt gctgaattcg tacacctaag gaggtgtaca gcatgaaacg
     1501 taaactcgag gaacttttca cgggggtcgt cccaattctg gtagaattag acggtgatgt
     1561 aaacgggcac aagtttagcg tcagcggaga gggcgagggg gacgccacct acggtaaact
     1621 tacccttaag tttatttgca cgacgggcaa attacccgtt ccgtggccaa cgctcgtcac
     1681 tactttcggc tacggcgttc agtgcttcgc gagatatccg gatcacatga agcagcacga
     1741 tttcttcaag tcggccatgc cagagggtta cgttcaggag agaactatat ttttcaagga
     1801 cgacggcaat tacaagacac gcgccgaagt caagttcgaa ggcgataccc tggttaaccg
     1861 tatcgagtta aaaggtatcg attttaaaga agacggaaac attctcggtc acaaactcga
     1921 atacaactat aacagccata atgtctacat tatggctgac aaacagaaga acggcattaa
     1981 ggttaacttc aaaattcgcc acaacatcga agacggtagc gtccaactag cagaccatta
     2041 tcaacaaaat actcctatcg gcgacggccc agtcctttta ccagacaacc attaccttag
     2101 cacacaaagc gcccttagca aagatcccaa cgagaagcgc gaccacatgg tccttctcga
     2161 atttgtcaca gcggcaggga tcacccacgg catggacgaa ctctacaaat aataattaat
     2221 taaaaaaaaa aaccccgccc ctgacagggc ggggtttttt tttctaatat gcggccgcca
     2281 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     2341 ggtgaacgct ctcctgagta ggacaaatcc gccgccctag agcatgc
//
