LOCUS       sequence-109551-        2392 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid is designed to validate the biophysical model of 
            translational coupling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Tian T, Salis HM
  TITLE     A predictive biophysical model of translational coupling to 
            coordinate and control protein expression in bacterial operons.
  JOURNAL   Nucleic Acids Res. 2015 Aug 18;43(14):7137-51. doi: 
            10.1093/nar/gkv635. Epub 2015 Jun 27.
  PUBMED    26117546
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkv635"; journalName: "Nucleic Acids Res"; date: 
            "2015-08-18- 18"; volume: "43"; issue: "14"; pages: "7137-51"
COMMENT     Sequence Label: pTd-4-15-13-6
FEATURES             Location/Qualifiers
     source          1..2392
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             779..787
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             791..1468
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MKASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIK
                     MRLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     RBS             1482..1490
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      2287..2373
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tctagaatta tcaaaaacta atatcaaata ggaaggtata catggtcgac aagggcgaag
       61 aactctttac cggcgtcgtc ccgatcctgg tagagcttga cggcgatgtc aacggtcata
      121 agtttagcgt tagcggcgaa ggcgagggcg acgcgacgta cggtaaatta accttaaaat
      181 tcatctgtac aaccggtaaa ttaccggtac cgtggcccac gttagttacc acccttacct
      241 ggggcgtcca gtgcttcgcg cgctatccag accacatgaa gcaacacgat ttcttcaaat
      301 cggctatgcc cgaaggctat gtacaggagc gcaccatctt cttcaaggac gacggtaatt
      361 ataaaacccg cgcagaagtt aagttcgagg gcgatacgct cgttaatcgc atcgaactca
      421 aaggtatcga cttcaaagag gacggcaaca ttttaggtca caagttagag tataacgcga
      481 tttccgataa cgtctacatt accgccgata aacaaaagaa cggtatcaaa gcaaacttta
      541 agattcgtca taacatcgag gacggctcgg tacagctcgc cgaccattac caacagaata
      601 cgccgatcgg cgacggtccg gtcctcctcc cggacaatca ttatctcagc acccagagcg
      661 cgctctccaa agatccaaac gagaagcgcg accacatggt ccttctcgaa tttgtaacgg
      721 cggcaggcat caccctcggt atggacgaac tctataagga cgtcaaaaaa tacctccgta
      781 aggaggtatc atgaaagcga gctccgaaga cgttatcaaa gaatttatgc gttttaaagt
      841 tcgcatggag ggctcggtta acggtcacga gttcgaaatc gaaggggaag gcgaaggtcg
      901 tccgtacgaa ggtacccaga ccgctaaact caaagttacc aaaggcggtc ccttaccgtt
      961 cgcttgggat attttaagcc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc
     1021 ggcggacatc ccggactacc ttaaattatc cttccccgaa gggtttaagt gggagcgcgt
     1081 tatgaacttc gaggacggcg gcgtcgttac cgttacccag gactcctccc ttcaagacgg
     1141 cgagttcatc tacaaagtta aacttcgcgg taccaacttc ccatccgatg gtccggtcat
     1201 gcagaagaag accatgggtt gggaagcttc caccgagcgc atgtatccgg aggacggcgc
     1261 tttaaaaggc gaaatcaaga tgcgcttaaa gttaaaagac ggcggtcact acgacgcaga
     1321 agttaaaacg acgtacatgg ccaagaaacc ggttcagtta ccgggcgctt acaaaaccga
     1381 catcaaactc gacatcacca gccacaacga agactacacc atcgtcgaac agtacgaacg
     1441 cgcagaaggt cgtcacagca ccggtgctga attcgtacac ctaaggaggt gtacagcatg
     1501 aaacgtaaac tcgaggaact tttcacgggg gtcgtcccaa ttctggtaga attagacggt
     1561 gatgtaaacg ggcacaagtt tagcgtcagc ggagagggcg agggggacgc cacctacggt
     1621 aaacttaccc ttaagtttat ttgcacgacg ggcaaattac ccgttccgtg gccaacgctc
     1681 gtcactactt tcggctacgg cgttcagtgc ttcgcgagat atccggatca catgaagcag
     1741 cacgatttct tcaagtcggc catgccagag ggttacgttc aggagagaac tatatttttc
     1801 aaggacgacg gcaattacaa gacacgcgcc gaagtcaagt tcgaaggcga taccctggtt
     1861 aaccgtatcg agttaaaagg tatcgatttt aaagaagacg gaaacattct cggtcacaaa
     1921 ctcgaataca actataacag ccataatgtc tacattatgg ctgacaaaca gaagaacggc
     1981 attaaggtta acttcaaaat tcgccacaac atcgaagacg gtagcgtcca actagcagac
     2041 cattatcaac aaaatactcc tatcggcgac ggcccagtcc ttttaccaga caaccattac
     2101 cttagcacac aaagcgccct tagcaaagat cccaacgaga agcgcgacca catggtcctt
     2161 ctcgaatttg tcacagcggc agggatcacc cacggcatgg acgaactcta caaataataa
     2221 ttaattaaaa aaaaaaaccc cgcccctgac agggcggggt ttttttttct aatatgcggc
     2281 cgccatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     2341 ttgtcggtga acgctctcct gagtaggaca aatccgccgc cctagagcat gc
//
