LOCUS       sequence-109554-        2388 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid is designed to validate the biophysical model of 
            translational coupling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Tian T, Salis HM
  TITLE     A predictive biophysical model of translational coupling to 
            coordinate and control protein expression in bacterial operons.
  JOURNAL   Nucleic Acids Res. 2015 Aug 18;43(14):7137-51. doi: 
            10.1093/nar/gkv635. Epub 2015 Jun 27.
  PUBMED    26117546
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkv635"; journalName: "Nucleic Acids Res"; date: 
            "2015-08-18- 18"; volume: "43"; issue: "14"; pages: "7137-51"
COMMENT     Sequence Label: pTd-4-15-13-9
FEATURES             Location/Qualifiers
     source          1..2388
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             775..783
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             787..1464
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MKASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIK
                     MRLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     RBS             1478..1486
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      2283..2369
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tctagacgaa atagatctaa atagggaggc aggaaaaatg gtcgacaagg gcgaagaact
       61 ctttaccggc gtcgtcccga tcctggtaga gcttgacggc gatgtcaacg gtcataagtt
      121 tagcgttagc ggcgaaggcg agggcgacgc gacgtacggt aaattaacct taaaattcat
      181 ctgtacaacc ggtaaattac cggtaccgtg gcccacgtta gttaccaccc ttacctgggg
      241 cgtccagtgc ttcgcgcgct atccagacca catgaagcaa cacgatttct tcaaatcggc
      301 tatgcccgaa ggctatgtac aggagcgcac catcttcttc aaggacgacg gtaattataa
      361 aacccgcgca gaagttaagt tcgagggcga tacgctcgtt aatcgcatcg aactcaaagg
      421 tatcgacttc aaagaggacg gcaacatttt aggtcacaag ttagagtata acgcgatttc
      481 cgataacgtc tacattaccg ccgataaaca aaagaacggt atcaaagcaa actttaagat
      541 tcgtcataac atcgaggacg gctcggtaca gctcgccgac cattaccaac agaatacgcc
      601 gatcggcgac ggtccggtcc tcctcccgga caatcattat ctcagcaccc agagcgcgct
      661 ctccaaagat ccaaacgaga agcgcgacca catggtcctt ctcgaatttg taacggcggc
      721 aggcatcacc ctcggtatgg acgaactcta taaggacgtc aaaaaatacc tccgtaagga
      781 ggtatcatga aagcgagctc cgaagacgtt atcaaagaat ttatgcgttt taaagttcgc
      841 atggagggct cggttaacgg tcacgagttc gaaatcgaag gggaaggcga aggtcgtccg
      901 tacgaaggta cccagaccgc taaactcaaa gttaccaaag gcggtccctt accgttcgct
      961 tgggatattt taagcccgca gttccagtac ggttccaaag cttacgttaa acacccggcg
     1021 gacatcccgg actaccttaa attatccttc cccgaagggt ttaagtggga gcgcgttatg
     1081 aacttcgagg acggcggcgt cgttaccgtt acccaggact cctcccttca agacggcgag
     1141 ttcatctaca aagttaaact tcgcggtacc aacttcccat ccgatggtcc ggtcatgcag
     1201 aagaagacca tgggttggga agcttccacc gagcgcatgt atccggagga cggcgcttta
     1261 aaaggcgaaa tcaagatgcg cttaaagtta aaagacggcg gtcactacga cgcagaagtt
     1321 aaaacgacgt acatggccaa gaaaccggtt cagttaccgg gcgcttacaa aaccgacatc
     1381 aaactcgaca tcaccagcca caacgaagac tacaccatcg tcgaacagta cgaacgcgca
     1441 gaaggtcgtc acagcaccgg tgctgaattc gtacacctaa ggaggtgtac agcatgaaac
     1501 gtaaactcga ggaacttttc acgggggtcg tcccaattct ggtagaatta gacggtgatg
     1561 taaacgggca caagtttagc gtcagcggag agggcgaggg ggacgccacc tacggtaaac
     1621 ttacccttaa gtttatttgc acgacgggca aattacccgt tccgtggcca acgctcgtca
     1681 ctactttcgg ctacggcgtt cagtgcttcg cgagatatcc ggatcacatg aagcagcacg
     1741 atttcttcaa gtcggccatg ccagagggtt acgttcagga gagaactata tttttcaagg
     1801 acgacggcaa ttacaagaca cgcgccgaag tcaagttcga aggcgatacc ctggttaacc
     1861 gtatcgagtt aaaaggtatc gattttaaag aagacggaaa cattctcggt cacaaactcg
     1921 aatacaacta taacagccat aatgtctaca ttatggctga caaacagaag aacggcatta
     1981 aggttaactt caaaattcgc cacaacatcg aagacggtag cgtccaacta gcagaccatt
     2041 atcaacaaaa tactcctatc ggcgacggcc cagtcctttt accagacaac cattacctta
     2101 gcacacaaag cgcccttagc aaagatccca acgagaagcg cgaccacatg gtccttctcg
     2161 aatttgtcac agcggcaggg atcacccacg gcatggacga actctacaaa taataattaa
     2221 ttaaaaaaaa aaaccccgcc cctgacaggg cggggttttt ttttctaata tgcggccgcc
     2281 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     2341 cggtgaacgc tctcctgagt aggacaaatc cgccgcccta gagcatgc
//
