LOCUS       sequence-109555-        2391 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid is designed to validate the biophysical model of 
            translational coupling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Tian T, Salis HM
  TITLE     A predictive biophysical model of translational coupling to 
            coordinate and control protein expression in bacterial operons.
  JOURNAL   Nucleic Acids Res. 2015 Aug 18;43(14):7137-51. doi: 
            10.1093/nar/gkv635. Epub 2015 Jun 27.
  PUBMED    26117546
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkv635"; journalName: "Nucleic Acids Res"; date: 
            "2015-08-18- 18"; volume: "43"; issue: "14"; pages: "7137-51"
COMMENT     Sequence Label: pTd-4-15-13-10
FEATURES             Location/Qualifiers
     source          1..2391
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             778..786
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             790..1467
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MKASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIK
                     MRLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     RBS             1481..1489
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      2286..2372
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tctagaaagc aaacaagcaa atatcaggag gaggtatata atggtcgaca agggcgaaga
       61 actctttacc ggcgtcgtcc cgatcctggt agagcttgac ggcgatgtca acggtcataa
      121 gtttagcgtt agcggcgaag gcgagggcga cgcgacgtac ggtaaattaa ccttaaaatt
      181 catctgtaca accggtaaat taccggtacc gtggcccacg ttagttacca cccttacctg
      241 gggcgtccag tgcttcgcgc gctatccaga ccacatgaag caacacgatt tcttcaaatc
      301 ggctatgccc gaaggctatg tacaggagcg caccatcttc ttcaaggacg acggtaatta
      361 taaaacccgc gcagaagtta agttcgaggg cgatacgctc gttaatcgca tcgaactcaa
      421 aggtatcgac ttcaaagagg acggcaacat tttaggtcac aagttagagt ataacgcgat
      481 ttccgataac gtctacatta ccgccgataa acaaaagaac ggtatcaaag caaactttaa
      541 gattcgtcat aacatcgagg acggctcggt acagctcgcc gaccattacc aacagaatac
      601 gccgatcggc gacggtccgg tcctcctccc ggacaatcat tatctcagca cccagagcgc
      661 gctctccaaa gatccaaacg agaagcgcga ccacatggtc cttctcgaat ttgtaacggc
      721 ggcaggcatc accctcggta tggacgaact ctataaggac gtcaaaaaat acctccgtaa
      781 ggaggtatca tgaaagcgag ctccgaagac gttatcaaag aatttatgcg ttttaaagtt
      841 cgcatggagg gctcggttaa cggtcacgag ttcgaaatcg aaggggaagg cgaaggtcgt
      901 ccgtacgaag gtacccagac cgctaaactc aaagttacca aaggcggtcc cttaccgttc
      961 gcttgggata ttttaagccc gcagttccag tacggttcca aagcttacgt taaacacccg
     1021 gcggacatcc cggactacct taaattatcc ttccccgaag ggtttaagtg ggagcgcgtt
     1081 atgaacttcg aggacggcgg cgtcgttacc gttacccagg actcctccct tcaagacggc
     1141 gagttcatct acaaagttaa acttcgcggt accaacttcc catccgatgg tccggtcatg
     1201 cagaagaaga ccatgggttg ggaagcttcc accgagcgca tgtatccgga ggacggcgct
     1261 ttaaaaggcg aaatcaagat gcgcttaaag ttaaaagacg gcggtcacta cgacgcagaa
     1321 gttaaaacga cgtacatggc caagaaaccg gttcagttac cgggcgctta caaaaccgac
     1381 atcaaactcg acatcaccag ccacaacgaa gactacacca tcgtcgaaca gtacgaacgc
     1441 gcagaaggtc gtcacagcac cggtgctgaa ttcgtacacc taaggaggtg tacagcatga
     1501 aacgtaaact cgaggaactt ttcacggggg tcgtcccaat tctggtagaa ttagacggtg
     1561 atgtaaacgg gcacaagttt agcgtcagcg gagagggcga gggggacgcc acctacggta
     1621 aacttaccct taagtttatt tgcacgacgg gcaaattacc cgttccgtgg ccaacgctcg
     1681 tcactacttt cggctacggc gttcagtgct tcgcgagata tccggatcac atgaagcagc
     1741 acgatttctt caagtcggcc atgccagagg gttacgttca ggagagaact atatttttca
     1801 aggacgacgg caattacaag acacgcgccg aagtcaagtt cgaaggcgat accctggtta
     1861 accgtatcga gttaaaaggt atcgatttta aagaagacgg aaacattctc ggtcacaaac
     1921 tcgaatacaa ctataacagc cataatgtct acattatggc tgacaaacag aagaacggca
     1981 ttaaggttaa cttcaaaatt cgccacaaca tcgaagacgg tagcgtccaa ctagcagacc
     2041 attatcaaca aaatactcct atcggcgacg gcccagtcct tttaccagac aaccattacc
     2101 ttagcacaca aagcgccctt agcaaagatc ccaacgagaa gcgcgaccac atggtccttc
     2161 tcgaatttgt cacagcggca gggatcaccc acggcatgga cgaactctac aaataataat
     2221 taattaaaaa aaaaaacccc gcccctgaca gggcggggtt tttttttcta atatgcggcc
     2281 gccatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     2341 tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc ctagagcatg c
//
