LOCUS       sequence-109560-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses OmpR constitutively from PompB97 and sfGFP under the 
            PompF146 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Schmidl SR, Sheth RU, Wu A, Tabor JJ
  TITLE     Refactoring and optimization of light-switchable escherichia coli 
            two-component systems.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. 
            Epub 2014 Oct 11.
  PUBMED    25250630
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500273n";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "820-31"
COMMENT     Sequence Label: pSR59.4
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..32
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(36..79)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 attaacctat aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc
       61 tttcgctaag gatgatttct ggttccttaa attttacttt tggttacata ttttttcttt
      121 ttgaaaccaa atctttatct ttgtagcact ttcacggtag cgaaacgtta gtttgaatgg
      181 aaagatgcct gcaggtgtca taaaaaaaac catgagggta ataaataatg cgtaaaggcg
      241 aagagctgtt cactggtgtc gtccctattc tggtggaact ggatggtgat gtcaacggtc
      301 ataagttttc cgtgcgtggc gagggtgaag gtgacgcaac taatggtaaa ctgacgctga
      361 agttcatctg tactactggt aaactgccgg taccttggcc gactctggta acgacgctga
      421 cttatggtgt tcagtgcttt gctcgttatc cggaccatat gaagcagcat gacttcttca
      481 agtccgccat gccggaaggc tatgtgcagg aacgcacgat ttcctttaag gatgacggca
      541 cgtacaaaac gcgtgcggaa gtgaaatttg aaggcgatac cctggtaaac cgcattgagc
      601 tgaaaggcat tgactttaaa gaagacggca atatcctggg ccataagctg gaatacaatt
      661 ttaacagcca caatgtttac atcaccgccg ataaacaaaa aaatggcatt aaagcgaatt
      721 ttaaaattcg ccacaacgtg gaggatggca gcgtgcagct ggctgatcac taccagcaaa
      781 acactccaat cggtgatggt cctgttctgc tgccagacaa tcactatctg agcacgcaaa
      841 gcgttctgtc taaagatccg aacgagaaac gcgatcatat ggttctgctg gagttcgtaa
      901 ccgca
//
